Detailed information of TA system TA149681

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4244914..4245749
• Replicon: chromosome
• Accession: NZ_CP048603
• Organism: Escherichia coli strain ChlosBP-23-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q707F1
• Locus tag: G3M71_RS20335
• Protein ID: WP_001094445.1
• Coordinates: 4245372..4245749 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: G3M71_RS20330
• Protein ID: WP_024174331.1
• Coordinates: 4244914..4245282 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M71_RS20305	4242339..4242572	+	234	WP_001117568.1	DUF905 family protein	-
G3M71_RS20310	4242663..4243481	+	819	WP_001234749.1	DUF945 domain-containing protein	-
G3M71_RS20315	4243573..4244058	+	486	WP_000214415.1	antirestriction protein	-
G3M71_RS20320	4244074..4244550	+	477	WP_001186725.1	RadC family protein	-
G3M71_RS20325	4244619..4244840	+	222	WP_000692346.1	DUF987 domain-containing protein	-
G3M71_RS20330	4244914..4245282	+	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G3M71_RS20335	4245372..4245749	+	378	WP_001094445.1	TA system toxin CbtA family protein	Toxin
G3M71_RS20340	4245746..4246234	+	489	WP_000761718.1	hypothetical protein	-
G3M71_RS20345	4246246..4246443	+	198	WP_000838536.1	DUF957 domain-containing protein	-
G3M71_RS20350	4246528..4247370	+	843	WP_001280501.1	DUF4942 domain-containing protein	-
G3M71_RS20355	4248119..4249657	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	papD / papC	4198179..4257470	59291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14194.10 Da
• Isoelectric Point: 6.8518

>T149681 WP_001094445.1 NZ_CP048603:4245372-4245749 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T149681 NZ_CP064217:879144-879551 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT149681 WP_024174331.1 NZ_CP048603:4244914-4245282 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT149681 NZ_CP064217:878897-879163 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A454A7U6