Detailed information of TA system TA149680

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4021069..4021852
• Replicon: chromosome
• Accession: NZ_CP048603
• Organism: Escherichia coli strain ChlosBP-23-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: G3M71_RS19240
• Protein ID: WP_096986021.1
• Coordinates: 4021069..4021446 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: G3M71_RS19245
• Protein ID: WP_204261138.1
• Coordinates: 4021493..4021852 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M71_RS19210	4016846..4017025	+	180	WP_001298063.1	hypothetical protein	-
G3M71_RS19215	4017424..4019460	-	2037	WP_163388739.1	hypothetical protein	-
G3M71_RS19225	4020413..4020571	-	159	WP_001467148.1	hypothetical protein	-
G3M71_RS19230	4020671..4020847	-	177	WP_163388834.1	DUF957 domain-containing protein	-
G3M71_RS19235	4020923..4021072	-	150	Protein_3761	hypothetical protein	-
G3M71_RS19240	4021069..4021446	-	378	WP_096986021.1	TA system toxin CbtA family protein	Toxin
G3M71_RS19245	4021493..4021852	-	360	WP_204261138.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G3M71_RS19250	4021932..4022153	-	222	WP_000692303.1	DUF987 domain-containing protein	-
G3M71_RS19255	4022222..4022698	-	477	WP_163388740.1	RadC family protein	-
G3M71_RS19260	4022714..4022884	-	171	Protein_3766	antirestriction protein	-
G3M71_RS19265	4023065..4023280	+	216	WP_001327840.1	hypothetical protein	-
G3M71_RS19270	4023348..4023545	+	198	WP_001550537.1	AlpA family transcriptional regulator	-
G3M71_RS19275	4023863..4024501	-	639	WP_000684813.1	RpiB/LacA/LacB family sugar-phosphate isomerase	-
G3M71_RS19280	4024743..4025912	+	1170	WP_163388741.1	oligogalacturonate lyase	-
G3M71_RS19285	4025945..4026745	+	801	WP_000345753.1	gluconate 5-dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4008055..4055051	46996

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (14-112)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14110.11 Da
• Isoelectric Point: 7.2652

>T149680 WP_096986021.1 NZ_CP048603:c4021446-4021069 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T149680 NZ_CP048603:c4021446-4021069 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13253.07 Da
• Isoelectric Point: 6.3140

>AT149680 WP_204261138.1 NZ_CP048603:c4021852-4021493 [Escherichia coli]
VSDTLPDDNHDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQAFPLLMKQLELMLTSGELNPR
HQHTVTLYAKGLACEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 360 bp

>AT149680 NZ_CP048603:c4021852-4021493 [Escherichia coli]
GTGTCAGACACACTCCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGCGC
CCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGTTCAGAGATGCGGATG
CGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGTGAACTGAATCCCCGC
CATCAGCATACCGTGACACTGTATGCGAAGGGGCTGGCCTGTGAAGCCGACACCCTCGGCTCCTGTGGTTACGTTTATAT
GGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA