Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3845283..3845833 Replicon chromosome
Accession NZ_CP048603
Organism Escherichia coli strain ChlosBP-23-1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag G3M71_RS18405 Protein ID WP_001160966.1
Coordinates 3845283..3845597 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag G3M71_RS18410 Protein ID WP_000125566.1
Coordinates 3845600..3845833 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M71_RS18380 3840958..3842244 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
G3M71_RS18385 3842244..3843233 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
G3M71_RS18390 3843230..3844051 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
G3M71_RS18395 3844054..3844431 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
G3M71_RS18400 3844438..3845286 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
G3M71_RS18405 3845283..3845597 - 315 WP_001160966.1 CcdB family protein Toxin
G3M71_RS18410 3845600..3845833 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
G3M71_RS18415 3845919..3846398 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
G3M71_RS18420 3846590..3848452 - 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
G3M71_RS18425 3848445..3848975 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
G3M71_RS18430 3849083..3850414 - 1332 WP_001183207.1 MFS transporter -
G3M71_RS18435 3850473..3850760 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T149675 WP_001160966.1 NZ_CP048603:c3845597-3845283 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T149675 NZ_CP064214:c1011-862 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT149675 WP_000125566.1 NZ_CP048603:c3845833-3845600 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT149675 NZ_CP064214:1056-1115 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References