Detailed information of TA system TA149659

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 1175984..1176717
• Replicon: chromosome
• Accession: NZ_CP048603
• Organism: Escherichia coli strain ChlosBP-23-1

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: G3M71_RS05590
• Protein ID: WP_128334586.1
• Coordinates: 1175984..1176313 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A443WPQ4
• Locus tag: G3M71_RS05595
• Protein ID: WP_032745872.1
• Coordinates: 1176352..1176717 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M71_RS05575	1171362..1172810	-	1449	WP_163388457.1	NADP-dependent succinate-semialdehyde dehydrogenase	-
G3M71_RS05580	1172833..1174101	-	1269	WP_000271981.1	L-2-hydroxyglutarate oxidase	-
G3M71_RS05585	1174121..1175098	-	978	WP_021520984.1	carbon starvation induced protein CsiD	-
G3M71_RS05590	1175984..1176313	-	330	WP_128334586.1	TA system toxin CbtA family protein	Toxin
G3M71_RS05595	1176352..1176717	-	366	WP_032745872.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G3M71_RS05600	1176743..1176964	-	222	WP_014226747.1	DUF987 domain-containing protein	-
G3M71_RS05605	1176961..1177503	-	543	WP_128334587.1	DNA repair protein RadC	-
G3M71_RS05610	1177516..1177959	-	444	WP_128334588.1	antirestriction protein	-
G3M71_RS05615	1177990..1178811	-	822	WP_128334589.1	DUF945 domain-containing protein	-
G3M71_RS05620	1178910..1179140	-	231	WP_014226751.1	DUF905 domain-containing protein	-
G3M71_RS05625	1179212..1179661	-	450	WP_032745015.1	hypothetical protein	-
G3M71_RS05630	1179658..1180110	-	453	WP_163388458.1	hypothetical protein	-
G3M71_RS05635	1180147..1180716	-	570	WP_032950001.1	hypothetical protein	-
G3M71_RS05640	1180716..1181420	-	705	WP_163388459.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	luxS	1132108..1177959	45851

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12377.16 Da
• Isoelectric Point: 6.8685

>T149659 WP_128334586.1 NZ_CP048603:c1176313-1175984 [Escherichia coli]
MQTISSHPTRATQPCLSPVEIWQLLLTHLLSQHYGLTLNDTPFIDETTIQEHIDAGISLSDAVNFLVEKYGLVRIDRKGF
SWQEQTPYLSVVDILRARRSTGLLKTNVK

Nucleotide

• Length: 330 bp

>T149659 NZ_CP064211:354089-354436 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13514.33 Da
• Isoelectric Point: 6.3162

>AT149659 WP_032745872.1 NZ_CP048603:c1176717-1176352 [Escherichia coli]
MNNHSESGTKPENPAIQQWGLKRDITPCFGARLVQEGNRLHYLADRAGFSGAFTDDVAMRLDQAFPLMMKQLELMLTSGE
LNPRHAHCVTLYHNGLTCEADTLGSCGYVYIAIYPEQTKPQ

Nucleotide

• Length: 366 bp

>AT149659 NZ_CP064211:354436-354735 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A443WPQ4