Detailed information of TA system TA149651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Phd(antitoxin)
• Location: 1657244..1657791
• Replicon: chromosome
• Accession: NZ_CP048602
• Organism: Halomonas piezotolerans strain NBT06E8

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GYM47_RS07575
• Protein ID: WP_153843913.1
• Coordinates: 1657489..1657791 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U7FV42
• Locus tag: GYM47_RS07570
• Protein ID: WP_022990189.1
• Coordinates: 1657244..1657501 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYM47_RS07540	1652347..1653417	+	1071	WP_153843800.1	RelA/SpoT domain-containing protein	-
GYM47_RS07545	1653509..1654183	+	675	WP_196781585.1	hypothetical protein	-
GYM47_RS07550	1654280..1655224	-	945	WP_153844212.1	IS5 family transposase	-
GYM47_RS07555	1655401..1656084	+	684	WP_153843916.1	hypothetical protein	-
GYM47_RS07560	1656180..1656548	+	369	WP_153843915.1	DUF3703 domain-containing protein	-
GYM47_RS07565	1656635..1657105	+	471	WP_153843914.1	hypothetical protein	-
GYM47_RS07570	1657244..1657501	+	258	WP_022990189.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GYM47_RS07575	1657489..1657791	+	303	WP_153843913.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GYM47_RS07580	1657856..1658467	+	612	Protein_1468	5'-nucleotidase	-
GYM47_RS07590	1658992..1659513	+	522	WP_153843912.1	GNAT family N-acetyltransferase	-
GYM47_RS07595	1659565..1659975	+	411	WP_153843911.1	thiol-disulfide oxidoreductase DCC family protein	-
GYM47_RS07600	1660007..1660906	-	900	WP_153843910.1	LysR family transcriptional regulator	-
GYM47_RS07605	1661062..1662513	+	1452	WP_153843909.1	3-isopropylmalate dehydratase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11506.20 Da
• Isoelectric Point: 4.5332

>T149651 WP_153843913.1 NZ_CP048602:1657489-1657791 [Halomonas piezotolerans]
MAEVIWTEPALQELDAIAEYIALDNPAAASHLVKEVFDKTERLADFPQSGRIPPELPNSVYREVVVPPCRIFYREDGQRV
LVLYVMREERQLRAYMLGNS

Nucleotide

• Length: 303 bp

>T149651 NZ_CP064208:4036076-4036294 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9887.41 Da
• Isoelectric Point: 8.5042

>AT149651 WP_022990189.1 NZ_CP048602:1657244-1657501 [Halomonas piezotolerans]
MKVELVTNLKRQATKILADLHLSKEPVLITEHGQPSAYLVDVQDYEFMQRRLELLEGLSRGERAVLEGRTYSQSEAREKM
SKWLK

Nucleotide

• Length: 258 bp

>AT149651 NZ_CP064208:4035675-4036049 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1I1I6C4