Detailed information of TA system TA149644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapD-relB/-
• Location: 503256..503913
• Replicon: chromosome
• Accession: NZ_CP048599
• Organism: Helicobacter pylori strain GCT 97

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: T5D7N9
• Locus tag: G3M69_RS02345
• Protein ID: WP_000271050.1
• Coordinates: 503629..503913 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: G3M69_RS02340
• Protein ID: WP_078258280.1
• Coordinates: 503256..503648 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M69_RS02315	498256..499368	+	1113	WP_163562906.1	hydrogenase formation protein HypD	-
G3M69_RS02320	499335..499745	+	411	WP_163562907.1	hypothetical protein	-
G3M69_RS02335	500623..502863	+	2241	WP_163562908.1	Hop family adhesin BabA	-
G3M69_RS02340	503256..503648	+	393	WP_078258280.1	hypothetical protein	Antitoxin
G3M69_RS02345	503629..503913	+	285	WP_000271050.1	endoribonuclease VapD	Toxin
G3M69_RS02350	504113..504637	-	525	WP_001135623.1	acyl-CoA thioesterase	-
G3M69_RS02355	504777..505634	+	858	WP_163562909.1	SDR family oxidoreductase	-
G3M69_RS02360	505627..506607	+	981	WP_000921450.1	iron ABC transporter permease	-
G3M69_RS02365	506607..507374	+	768	WP_000242349.1	ABC transporter ATP-binding protein	-
G3M69_RS02370	507478..508551	+	1074	WP_205424861.1	glycosyltransferase family 8 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11188.74 Da
• Isoelectric Point: 4.8896

>T149644 WP_000271050.1 NZ_CP048599:503629-503913 [Helicobacter pylori]
MYALAFDLKIEILKKEYGEPYNKAYDDLRQELELLGFEWTQGSVYVNYSKENTLAQVYKAINKLSQIEWFKKSVRDIRAF
KVEDFSDFTEIVKS

Nucleotide

• Length: 285 bp

>T149644 NZ_CP064206:150552-151022 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 15285.30 Da
• Isoelectric Point: 7.1102

>AT149644 WP_078258280.1 NZ_CP048599:503256-503648 [Helicobacter pylori]
MPNTTAKKDYTKYSEKQLFNLIHQLERKIKKMQNDRVSFKEKMAKELEKRDQNFKDRIDALNELLQKISQAFDDKRDCCL
GHETPNIETQQAMRDALNGVNLTQIDSLDDLTNELKRENNKGFENVCFSV

Nucleotide

• Length: 393 bp

>AT149644 NZ_CP064206:150112-150555 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• PDB: 3UI3