Detailed information of TA system TA149632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 320497..320983
• Replicon: chromosome
• Accession: NZ_CP048589
• Organism: Agrobacterium tumefaciens strain But002

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A846L580
• Locus tag: FY129_RS01520
• Protein ID: WP_003503189.1
• Coordinates: 320497..320766 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A822V377
• Locus tag: FY129_RS01525
• Protein ID: WP_003503188.1
• Coordinates: 320750..320983 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY129_RS01485 (FY129_01490)	315559..316188	+	630	WP_003503196.1	DNA-3-methyladenine glycosylase I	-
FY129_RS01490 (FY129_01495)	316185..316631	+	447	WP_087743401.1	hypothetical protein	-
FY129_RS01495 (FY129_01500)	316647..317690	-	1044	WP_065694890.1	YeiH family protein	-
FY129_RS01500 (FY129_01505)	317772..318689	+	918	WP_026363679.1	LysR substrate-binding domain-containing protein	-
FY129_RS01505 (FY129_01510)	318860..319234	-	375	WP_080865877.1	DUF305 domain-containing protein	-
FY129_RS01510 (FY129_01515)	319292..319684	-	393	WP_233284482.1	hypothetical protein	-
FY129_RS01515 (FY129_01520)	319726..320490	-	765	WP_113082707.1	hypothetical protein	-
FY129_RS01520 (FY129_01525)	320497..320766	-	270	WP_003503189.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY129_RS01525 (FY129_01530)	320750..320983	-	234	WP_003503188.1	DUF6290 family protein	Antitoxin
FY129_RS01530 (FY129_01535)	321141..322664	+	1524	WP_080865881.1	histidine--tRNA ligase	-
FY129_RS01535 (FY129_01540)	322669..323037	+	369	WP_020010527.1	VOC family protein	-
FY129_RS01540 (FY129_01545)	323054..324181	+	1128	WP_087743414.1	ATP phosphoribosyltransferase regulatory subunit	-
FY129_RS01545 (FY129_01550)	324178..324870	+	693	WP_087743415.1	ATP phosphoribosyltransferase	-
FY129_RS01550 (FY129_01555)	324933..325379	-	447	WP_003503183.1	DoxX family protein	-
FY129_RS01555 (FY129_01560)	325536..325970	-	435	WP_003503182.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (6-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10341.95 Da
• Isoelectric Point: 10.6086

>T149632 WP_003503189.1 NZ_CP048589:c320766-320497 [Agrobacterium tumefaciens]
MIWKIEFQRAAVKQLGALTKSDANRIVSFLTDRIARDGNPRRTGAALQGSELGNFWRYRVGDYRIICDIQDHKLVVLVVE
IGHRREIYR

Nucleotide

• Length: 270 bp

>T149632 NZ_CP064203:709154-709336 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
CAAACACCCAACCAAAAAGGGATTAGTTACTATCCCGCATCCTAAAAAGGATTTACCAAGCGGAACTGTTAAAAGCATTT
TGAAGCAAGCGGGTCTAAAGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 9212.31 Da
• Isoelectric Point: 4.6044

>AT149632 WP_003503188.1 NZ_CP048589:c320983-320750 [Agrobacterium tumefaciens]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRQRIANKERTYTLEEVMRDLDLEN

Nucleotide

• Length: 234 bp

>AT149632 NZ_CP064203:709401-709805 [Acinetobacter baumannii]
ATGTTGTATCCAATTGCTATCGAGAGAGGTACAGACACCGAGGCCTTTGGTGTCACCGTTCCAGATATTCCAGGATGTTT
TAGCGCAGGCGATACATTAGAGGAAGCTATCGAGAACGTTAAAGAGGCAATTTCTGGCCACTTAGAAATCCTTGCTGAAG
ATGGAGAAGAAATTCCATTAGCATCTGACTTAGCAAAATTTGTAGATGATCCAGATTATAAAGGCATGATCTGGGCTGTT
ACTGAGGTGGATGTTAGTCGTTATCTTGGTAAGCCTGAAAAAATCAACGTAACTTTACCTAGCCGTTTAATTCGCAAGAT
TGATGATAATGTAGGTAAAGATAAAAGATTTAAAACTCGTTCTGCTTTTTTGGCCGCTGGTGCTGAAAAGCTACTACATG
CTTAA

Structures

Toxin

• AlphaFold DB: A0A846L580

Antitoxin

• AlphaFold DB: A0A822V377