Detailed information of TA system TA149631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 315779..316449
• Replicon: plasmid pAt1
• Accession: NZ_CP048586
• Organism: Agrobacterium pusense strain CC001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FY130_RS24835
• Protein ID: WP_262622794.1
• Coordinates: 315779..316198 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U4Q725
• Locus tag: FY130_RS24840
• Protein ID: WP_006699039.1
• Coordinates: 316195..316449 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY130_RS24810 (FY130_24600)	310888..312078	+	1191	WP_262622790.1	DUF932 domain-containing protein	-
FY130_RS24815 (FY130_24605)	312183..312437	+	255	WP_262622791.1	DUF6117 family protein	-
FY130_RS24820 (FY130_24610)	312566..313018	+	453	WP_262622792.1	hypothetical protein	-
FY130_RS24825 (FY130_24615)	313031..314068	+	1038	WP_107341275.1	toprim domain-containing protein	-
FY130_RS24830 (FY130_24620)	314398..315339	+	942	WP_262622793.1	DUF2493 domain-containing protein	-
FY130_RS24835 (FY130_24625)	315779..316198	-	420	WP_262622794.1	type II toxin-antitoxin system VapC family toxin	Toxin
FY130_RS24840 (FY130_24630)	316195..316449	-	255	WP_006699039.1	hypothetical protein	Antitoxin
FY130_RS24845 (FY130_24635)	316618..316848	+	231	WP_128636328.1	hypothetical protein	-
FY130_RS24850 (FY130_24640)	316900..317406	-	507	WP_262622795.1	RES domain-containing protein	-
FY130_RS24855 (FY130_24645)	317403..317888	-	486	WP_025555388.1	DUF2384 domain-containing protein	-
FY130_RS24860 (FY130_24650)	318294..318617	+	324	WP_262622796.1	DUF736 family protein	-
FY130_RS24865 (FY130_24655)	318674..319006	+	333	WP_262622797.1	hypothetical protein	-
FY130_RS24870 (FY130_24660)	319072..319350	-	279	WP_006699033.1	HU family DNA-binding protein	-
FY130_RS24875	319742..319906	+	165	WP_262622798.1	hypothetical protein	-
FY130_RS24880 (FY130_24665)	320045..320371	-	327	WP_262622799.1	WGR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd / htpB	1..426589	426589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15032.12 Da
• Isoelectric Point: 4.8728

>T149631 WP_262622794.1 NZ_CP048586:c316198-315779 [Agrobacterium pusense]
MILLDTNVVSETMKPFPSQMVQRWLDEQAAETLHLSSITIAEAIFGIRTMPDGQRKQRLAEALDVWIGLFEGRILPFDLD
AAKHYANLGSSARASGKGFPTPDGYIAAIAASKGFAVATRDASAFHAAGLEVIDPWTAE

Nucleotide

• Length: 420 bp

>T149631 NZ_CP064202:6-293 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9374.65 Da
• Isoelectric Point: 6.7270

>AT149631 WP_006699039.1 NZ_CP048586:c316449-316195 [Agrobacterium pusense]
VPAVTIRNIPEETHRAIKIRAAQHGRSAEAEMRDILEMAVRPATRLRLGTALAEASRRIGLTNEDVDALEQTFDRTPAHP
MSFE

Nucleotide

• Length: 255 bp

>AT149631 NZ_CP064202:280-594 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Antitoxin

• AlphaFold DB: U4Q725