Detailed information of TA system TA149630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 201006..201739
• Replicon: plasmid pAt1
• Accession: NZ_CP048586
• Organism: Agrobacterium pusense strain CC001

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: FY130_RS24235
• Protein ID: WP_262622992.1
• Coordinates: 201006..201305 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: FY130_RS24240
• Protein ID: WP_262622993.1
• Coordinates: 201401..201739 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY130_RS24200 (FY130_24015)	196324..196614	-	291	WP_100911704.1	co-chaperone GroES	-
FY130_RS24205 (FY130_24020)	197100..197327	-	228	WP_142188067.1	hypothetical protein	-
FY130_RS24210 (FY130_24025)	197324..197569	-	246	WP_262622988.1	hypothetical protein	-
FY130_RS24215 (FY130_24030)	197827..198093	-	267	WP_142188065.1	DUF1488 domain-containing protein	-
FY130_RS24220 (FY130_24035)	198093..198353	-	261	WP_142188064.1	hypothetical protein	-
FY130_RS24225 (FY130_24040)	198737..199597	+	861	WP_262622990.1	DUF1403 family protein	-
FY130_RS24230	199536..199691	+	156	WP_262622991.1	DUF1403 family protein	-
FY130_RS24235 (FY130_24045)	201006..201305	+	300	WP_262622992.1	BrnT family toxin	Toxin
FY130_RS24240 (FY130_24050)	201401..201739	+	339	WP_262622993.1	BrnA antitoxin family protein	Antitoxin
FY130_RS24245 (FY130_24055)	201842..202900	+	1059	WP_262622994.1	PAS domain S-box protein	-
FY130_RS24250 (FY130_24060)	203027..203287	+	261	WP_262622995.1	hypothetical protein	-
FY130_RS24255 (FY130_24065)	203304..203672	-	369	WP_262622996.1	hypothetical protein	-
FY130_RS24260 (FY130_24070)	205249..205389	+	141	Protein_211	Tn3 family transposase	-
FY130_RS24265 (FY130_24075)	205376..205474	+	99	Protein_212	transposase	-
FY130_RS24270 (FY130_24080)	205591..206400	-	810	WP_262622997.1	transglutaminase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd / htpB	1..426589	426589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (22-95)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (36-107)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11182.84 Da
• Isoelectric Point: 8.9696

>T149630 WP_262622992.1 NZ_CP048586:201006-201305 [Agrobacterium pusense]
MYPQQICVAKIFIVHTIYEIVWDEHKRQSNLTKHRLDFADLSIDFFAAAKVPPSEEGRSKAIGNFEGVTVIAVAFRPYGS
EALSVVSMRRANKAERNLS

Nucleotide

• Length: 300 bp

>T149630 NZ_CP064194:c3482718-3482329 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12665.47 Da
• Isoelectric Point: 8.6073

>AT149630 WP_262622993.1 NZ_CP048586:201401-201739 [Agrobacterium pusense]
MKELIAKGGVHQAEDGWWVDTATGEMIGPDSEIERPLEADEQAKMKPLREALPELAKSIDREVARRGRPKAQTHKTPVTI
RLDPEVVEHYKAMGKGWQSRINNDLRKLSGLR

Nucleotide

• Length: 339 bp

>AT149630 NZ_CP064194:c3482982-3482725 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA