Detailed information of TA system TA149629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 100614..101404
• Replicon: plasmid pAt1
• Accession: NZ_CP048586
• Organism: Agrobacterium pusense strain CC001

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: FY130_RS23750
• Protein ID: WP_262622957.1
• Coordinates: 100907..101404 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A7W4U0T2
• Locus tag: FY130_RS23745
• Protein ID: WP_044460092.1
• Coordinates: 100614..100910 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY130_RS23720 (FY130_23545)	96513..97226	+	714	WP_111814480.1	GntR family transcriptional regulator	-
FY130_RS23725 (FY130_23550)	97223..98143	+	921	WP_111814482.1	dihydrodipicolinate synthase family protein	-
FY130_RS23730 (FY130_23555)	98157..99296	+	1140	WP_111814484.1	mandelate racemase/muconate lactonizing enzyme family protein	-
FY130_RS23735	99738..99944	+	207	Protein_106	hypothetical protein	-
FY130_RS23740 (FY130_23560)	100098..100220	-	123	Protein_107	LacI family transcriptional regulator	-
FY130_RS23745 (FY130_23565)	100614..100910	+	297	WP_044460092.1	DUF1778 domain-containing protein	Antitoxin
FY130_RS23750 (FY130_23570)	100907..101404	+	498	WP_262622957.1	GNAT family N-acetyltransferase	Toxin
FY130_RS23755	101752..102048	-	297	Protein_110	GNAT family N-acetyltransferase	-
FY130_RS23760 (FY130_23575)	102078..102562	+	485	Protein_111	glutathione binding-like protein	-
FY130_RS23765 (FY130_23580)	102789..103247	-	459	WP_262622958.1	gamma carbonic anhydrase family protein	-
FY130_RS23770 (FY130_23585)	103273..103653	-	381	WP_184289874.1	nuclear transport factor 2 family protein	-
FY130_RS23775 (FY130_23590)	103746..104780	+	1035	WP_262622959.1	transcriptional regulator FtrA	-
FY130_RS23780 (FY130_23595)	104814..106013	-	1200	WP_262622960.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd / htpB	1..426589	426589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (16-90)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 17701.44 Da
• Isoelectric Point: 7.2441

>T149629 WP_262622957.1 NZ_CP048586:100907-101404 [Agrobacterium pusense]
VTLTFPAPLAEHHELAEFNSGVPELDDWLRRRARANQAGGASRTFVVCEGNRVVAYYALASGAVRTPEAPGRFKRNMPDP
ILIAVLGRLAIDLSYQGCGIGRALVRDAGLRLLNAAEILGIRGVLVHAISDDARAFYEAVGFMPSPSDPMMLMIGLKDLS
NALSA

Nucleotide

• Length: 498 bp

>T149629 NZ_CP064194:c2736794-2736612 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
CAAACACCCAACCAAAAAGGGATTAGTTACTATCCCGCATCCTAAAAAGGATTTACCAAGCGGAACTGTTAAAAGCATTT
TGAAGCAAGCGGGTCTAAAGTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11100.69 Da
• Isoelectric Point: 9.6895

>AT149629 WP_044460092.1 NZ_CP048586:100614-100910 [Agrobacterium pusense]
MPTSHTSKNLDASVPLNIRIKPATRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRTVFTVSPEAYAEYLTRLDAPAQ
SNERLKRTMSIKAPWDKA

Nucleotide

• Length: 297 bp

>AT149629 NZ_CP064194:c2736547-2736143 [Acinetobacter baumannii]
ATGTTGTATCCAATTGCTATCGAGAGAGGTACAGACACCGAGGCCTTTGGTGTCACCGTTCCAGATATTCCAGGATGTTT
TAGCGCAGGCGATACATTAGAGGAAGCTATCGAGAACGTTAAAGAGGCAATTTCTGGCCACTTAGAAATCCTTGCTGAAG
ATGGAGAAGAAATTCCATTAGCATCTGACTTAGCAAAATTTGTAGATGATCCAGATTATAAAGGCATGATCTGGGCTGTT
ACTGAGGTGGATGTTAGTCGTTATCTTGGTAAGCCTGAAAAAATCAACGTAACTTTACCTAGCCGTTTAATTCGCAAGAT
TGATGATAATGTAGGTAAAGATAAAAGATTTAAAACTCGTTCTGCTTTTTTGGCCGCTGGTGCTGAAAAGCTACTACATG
CTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7W4U0T2