Detailed information of TA system TA149626

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2058939..2059545
• Replicon: chromosome
• Accession: NZ_CP048584
• Organism: Agrobacterium pusense strain CC001

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7W4TY99
• Locus tag: FY130_RS10260
• Protein ID: WP_004443439.1
• Coordinates: 2058939..2059235 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: FY130_RS10265
• Protein ID: WP_004443440.1
• Coordinates: 2059240..2059545 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY130_RS10235 (FY130_10150)	2054728..2055621	-	894	WP_004443429.1	hemin ABC transporter substrate-binding protein	-
FY130_RS10240 (FY130_10155)	2055808..2056155	+	348	WP_004429958.1	antibiotic biosynthesis monooxygenase	-
FY130_RS10245 (FY130_10160)	2056165..2056689	+	525	WP_004443430.1	heme utilization cystosolic carrier protein HutX	-
FY130_RS10250 (FY130_10165)	2057025..2057801	+	777	WP_004443433.1	YbaY family lipoprotein	-
FY130_RS10255 (FY130_10170)	2058009..2058893	+	885	WP_004443436.1	formyltetrahydrofolate deformylase	-
FY130_RS10260 (FY130_10175)	2058939..2059235	+	297	WP_004443439.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY130_RS10265 (FY130_10180)	2059240..2059545	+	306	WP_004443440.1	putative addiction module antidote protein	Antitoxin
FY130_RS10270 (FY130_10185)	2059557..2060951	-	1395	WP_004443442.1	sensor histidine kinase	-
FY130_RS10275 (FY130_10190)	2060948..2061628	-	681	WP_004443444.1	response regulator transcription factor	-
FY130_RS10280 (FY130_10195)	2061710..2062777	+	1068	WP_006700422.1	ABC transporter substrate-binding protein	-
FY130_RS10285 (FY130_10200)	2063128..2064072	+	945	WP_004443448.1	tripartite tricarboxylate transporter substrate binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10923.61 Da
• Isoelectric Point: 10.2206

>T149626 WP_004443439.1 NZ_CP048584:2058939-2059235 [Agrobacterium pusense]
MLTIRETSEFADWLAGLRDLQARARIARRIYRLADGNPGDVKPVGEGVSELRIDFGPGYRVYFFRNGDVVIILLCGGDKA
SQSRDIAKAKKLARALKE

Nucleotide

• Length: 297 bp

>T149626 NZ_CP064192:224305-224559 [Saccharothrix sp. 6-C]
GTGAGGCTCGTCTTCGAAGAGCAGGGCTGGGAGGACTACACGTCCTGGCTCAAGAACGACCGCAAGATGCTCGCCCGGGT
CAACAAGCTCATCGAGGACGCGCTCCGGGATCCGTTCACCGGGATCGGCAAACCCGAGCCGCTGAAGTACCACCTGCCCG
GCGCTTGGTCGAGGCGGATCGATGACGAACACCGCCTCGTCTACCTGGTCGTCGACGACCAGGTCATCGTGCTCGCCGCT
CGGTACCACTACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10547.84 Da
• Isoelectric Point: 4.5580

>AT149626 WP_004443440.1 NZ_CP048584:2059240-2059545 [Agrobacterium pusense]
MPLVTRDYDAARYLDSDEALAAYIADATENGDAQELAHALGVVARAKGITDLSRQTGLPRQTLYKALSGEGNPELATIAK
VADALGYKLSLVAKSPADTAA

Nucleotide

• Length: 306 bp

>AT149626 NZ_CP064192:224027-224308 [Saccharothrix sp. 6-C]
ATGTCGATTACCGCGAGTGAGGCCCGCAAGGCTCTCTTCCCGCTGATCAAGCAGGTCAACGAAGACCACGAGGCCATCGA
GATCGTCTCCAAGCACGGCAATGCCGTGCTCGTCTCGGCCGAGGACTACGCCGCGCTCCGTGAAGGCTCTTACCTCTTGC
GCTCGCCCGCCAACGCGCGTCGCCTGCTCAAGGCCTACGAGAACGCTCTCGGCGGCGTCGGGCTCTCGGCGCGCGAGTTG
ATCGACTCTGAGGCCGCGGACGCGAAAGCCGGAGCCGAGTGA

Structures

Toxin

• AlphaFold DB: A0A7W4TY99