Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 2107694..2108300 Replicon chromosome
Accession NZ_CP048564
Organism Agrobacterium tumefaciens strain Gle002

Toxin (Protein)


Gene name PumA Uniprot ID A0A822UZY8
Locus tag FY133_RS10475 Protein ID WP_020011654.1
Coordinates 2107694..2107990 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A822V132
Locus tag FY133_RS10480 Protein ID WP_060723677.1
Coordinates 2107995..2108300 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FY133_RS10440 (FY133_10365) 2103289..2103636 + 348 WP_020809369.1 antibiotic biosynthesis monooxygenase -
FY133_RS10445 (FY133_10370) 2103646..2104170 + 525 WP_020809370.1 heme utilization cystosolic carrier protein HutX -
FY133_RS10450 (FY133_10375) 2104354..2104743 + 390 WP_240532256.1 hypothetical protein -
FY133_RS10455 (FY133_10380) 2104896..2105435 + 540 WP_060723673.1 DUF2975 domain-containing protein -
FY133_RS10460 (FY133_10385) 2105445..2105687 + 243 WP_060723674.1 helix-turn-helix transcriptional regulator -
FY133_RS10465 (FY133_10390) 2105781..2106557 + 777 WP_020809374.1 YbaY family lipoprotein -
FY133_RS10470 (FY133_10395) 2106764..2107648 + 885 WP_262563298.1 formyltetrahydrofolate deformylase -
FY133_RS10475 (FY133_10400) 2107694..2107990 + 297 WP_020011654.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FY133_RS10480 (FY133_10405) 2107995..2108300 + 306 WP_060723677.1 putative addiction module antidote protein Antitoxin
FY133_RS10485 (FY133_10410) 2108314..2109708 - 1395 WP_065695049.1 sensor histidine kinase -
FY133_RS10490 (FY133_10415) 2109705..2110385 - 681 WP_003491888.1 response regulator transcription factor -
FY133_RS10495 (FY133_10420) 2110455..2111534 + 1080 WP_080866414.1 ABC transporter substrate-binding protein -
FY133_RS10500 (FY133_10425) 2111960..2112904 + 945 WP_004429910.1 tripartite tricarboxylate transporter substrate binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-97)

Antitoxin

Cro/C1-type helix-turn-helix domain

(47-90)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 10888.61 Da        Isoelectric Point: 10.2733

>T149621 WP_020011654.1 NZ_CP048564:2107694-2107990 [Agrobacterium tumefaciens]
MLTIRETSEFADWLAGLRDVQARARIARRIYRLADGNPGDVKPVGEGVSELRIDLGPGYRVYFVRHGDVVIILLCGGDKA
SQPRDIARAKKLARALKE

Download         Length: 297 bp

>T149621 NZ_CP064191:306712-307002 [Rhizobium sp. 007]
ATGATTGAAGTTCGACTGACAGTCGTTTTTTCCCGATGGCTGGAAGATCTCAAAGACCGGCGCGCGCGTGACAGAGTTCT
CGCCAGATTGCGCCGTTTCGAATTAGAAAATTTCGGAGACGCAAAGCCCGTGGGGAGCGGGGTGAGCGAGGCACGAATAG
ATTATGGGCCGGGGTATCGCCTATATTTTGTGAGGCAGGCTGATGTTGTCGTGGTGATGCTGTGTGGTGGCGACAAAGCA
AGCCAGAAACGCGACATAGCGACGGCGAAGGCAATGGCGAAGGAGCTATAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 10576.90 Da        Isoelectric Point: 4.5580

>AT149621 WP_060723677.1 NZ_CP048564:2107995-2108300 [Agrobacterium tumefaciens]
MPLVTRDYDAARYLDSDEALAAYIADATESGDAQELAHALGVVARAKGMTDLSRQTGLPRQTLYKALSGEGNPELATIAK
VADALGYRLSLVAKPPADTAA

Download         Length: 306 bp

>AT149621 NZ_CP064191:307004-307312 [Rhizobium sp. 007]
ATGGCATTGGAAACAACGCGCTTCGATATTCAGAACTACATCAAAAGTCACGAGGATCAGACTGGCTATCTCGACGCTGT
TCTGGAGGATGGAGATCCTGCTCTTATTGCAGCGGCCATCGGCGATATTGCCCGCGCACGCGGCGCATCACAATTCGCGA
AAGACAGCGGACTCAGTCGTGAAACCATCTATAAGGTTTTTCGCCCAGGGGGAAACCCGACGCTCGATACGATCGCCAAA
GCGGTGAAAGTGCTTGGCCTGCGGCTGTCGCTGGTACCGGCATCCAACTCCACCAAAGGCCCGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822UZY8

Antitoxin

Source ID Structure
AlphaFold DB A0A822V132

References