Detailed information of TA system TA149619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 519944..520488
• Replicon: chromosome
• Accession: NZ_CP048564
• Organism: Agrobacterium tumefaciens strain Gle002

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: FY133_RS02550
• Protein ID: WP_026363704.1
• Coordinates: 520198..520488 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A822V4A8
• Locus tag: FY133_RS02545
• Protein ID: WP_003509084.1
• Coordinates: 519944..520195 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY133_RS02520 (FY133_02515)	515702..516382	+	681	WP_003509096.1	GntR family transcriptional regulator	-
FY133_RS02525 (FY133_02520)	516428..516904	+	477	WP_099085593.1	DUF1284 domain-containing protein	-
FY133_RS02530 (FY133_02525)	517084..518184	-	1101	WP_020811573.1	hypothetical protein	-
FY133_RS02535 (FY133_02530)	518150..518362	+	213	WP_003509088.1	hypothetical protein	-
FY133_RS02540 (FY133_02535)	518421..519566	+	1146	WP_020010628.1	site-specific DNA-methyltransferase	-
FY133_RS02545 (FY133_02540)	519944..520195	+	252	WP_003509084.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
FY133_RS02550 (FY133_02545)	520198..520488	+	291	WP_026363704.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY133_RS02555 (FY133_02550)	520530..521141	-	612	WP_003509082.1	HAD family phosphatase	-
FY133_RS02560 (FY133_02555)	521138..522250	-	1113	WP_099085591.1	A/G-specific adenine glycosylase	-
FY133_RS02565 (FY133_02560)	522378..522842	+	465	WP_003509080.1	hypothetical protein	-
FY133_RS02570 (FY133_02565)	522870..523391	+	522	WP_003509079.1	DUF721 domain-containing protein	-
FY133_RS02575 (FY133_02570)	523520..524200	+	681	WP_003509078.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (12-70)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11195.86 Da
• Isoelectric Point: 6.2182

>T149619 WP_026363704.1 NZ_CP048564:520198-520488 [Agrobacterium tumefaciens]
VGFRLSLLAEEDVIRIAEEGIAIFGLAQAQKYHRELYEIFELISLNPRMARERHELLPPLRIHRFRAHLVVYGVGADDDV
LIVRVRHAHEDWVNES

Nucleotide

• Length: 291 bp

>T149619 NZ_CP064189:247037-247438 [Rhizobium sp. 007]
ATGCCACGCTACATGCTCGATACCAATATGTGTATCTACCTGATGAAGAACCAGCCGGAGCAGGTCGCCCGCCGCTTTGC
CAGTTGCTATGTGGGTGACGTTGTGATGTCCGCCATCACCTTCGCGGAACTCGAGTACGGAGTTTCCGTATCCGATGATC
CAGCGCGGGAACTCGATAATCTTGCAGCCCTGGCCGAGCTGATCACGGTAGAGCCGTTCGGGATCGCGGCAGCGCGTGCC
TATGGTCCGGTTCGTCTGGCGACTCGGGACCGGAAGAAGGATCACCTGGACAAACTGATCGCTTCGCATGCAATCGCATT
GAACACCGTGCTCGTTACCAACAATACGCGAGACTTTCTGGCGTATCCCGGACTGAGGCTCGAAAACTGGCTTGACGCCT
GA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9339.55 Da
• Isoelectric Point: 9.7317

>AT149619 WP_003509084.1 NZ_CP048564:519944-520195 [Agrobacterium tumefaciens]
MATMNISLPAPMKEWVEAQSRTGRYSNASDYVRDLIRKDQERGEKIAAMQHFVDEGLQSGAGHRSQQALFAEAEARSMKR
KIG

Nucleotide

• Length: 252 bp

>AT149619 NZ_CP064189:246801-247037 [Rhizobium sp. 007]
ATGCACACCACCAAAGCTTTCAAGAACGGAAATTCCCAGGCCGTGCGCATCCCGGCCGAACTGGCCTTCGACCGGACCGA
TGTCGAGTTGGAGATCGAGCGTGTGGGCGACGAGCTTCGCATCCGTCCGGTGCATCGCTCCCTGGCCGGCGTTCTGGACG
TCTTTGCCCGCTTCTCGCCGGATTTCATGCGCGACGGAAGAGAGCCCCATGAGCAAGGCGAGCGGGACAAGATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A822V4A8