Detailed information of TA system TA149614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 324246..324726
• Replicon: chromosome
• Accession: NZ_CP048558
• Organism: Agrobacterium fabrum strain Kin001

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: FY134_RS01560
• Protein ID: WP_111805655.1
• Coordinates: 324246..324515 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A9CK21
• Locus tag: FY134_RS01565
• Protein ID: WP_010971060.1
• Coordinates: 324499..324726 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY134_RS01530 (FY134_01530)	319297..320073	-	777	WP_111805662.1	L,D-transpeptidase	-
FY134_RS01535 (FY134_01535)	320312..320941	+	630	WP_111805660.1	DNA-3-methyladenine glycosylase I	-
FY134_RS01540 (FY134_01540)	320938..321381	+	444	WP_111805659.1	hypothetical protein	-
FY134_RS01545 (FY134_01545)	321407..322450	-	1044	WP_035256280.1	YeiH family protein	-
FY134_RS01550 (FY134_01550)	322532..323449	+	918	WP_010971057.1	LysR family transcriptional regulator	-
FY134_RS01555 (FY134_01555)	323475..324239	-	765	WP_111805657.1	hypothetical protein	-
FY134_RS01560 (FY134_01560)	324246..324515	-	270	WP_111805655.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY134_RS01565 (FY134_01565)	324499..324726	-	228	WP_010971060.1	DUF6290 family protein	Antitoxin
FY134_RS01570 (FY134_01570)	324883..326406	+	1524	WP_111805653.1	histidine--tRNA ligase	-
FY134_RS01575 (FY134_01575)	326411..326779	+	369	WP_111805651.1	VOC family protein	-
FY134_RS01580 (FY134_01580)	326796..327923	+	1128	WP_006313159.1	ATP phosphoribosyltransferase regulatory subunit	-
FY134_RS01585 (FY134_01585)	327920..328612	+	693	WP_006313160.1	ATP phosphoribosyltransferase	-
FY134_RS01590 (FY134_01590)	328676..329122	-	447	WP_006313161.1	DoxX family protein	-
FY134_RS01595 (FY134_01595)	329278..329697	-	420	WP_230060738.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (6-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10741.44 Da
• Isoelectric Point: 9.9415

>T149614 WP_111805655.1 NZ_CP048558:c324515-324246 [Agrobacterium fabrum]
VIWTIEYHTLVQKEMRKINPEVRRRIRSFLHERLAGLDDPRQIGATLQGSELGNFWRYRVGDYRIICDIQDHKLVVLVVE
IGHRREIYR

Nucleotide

• Length: 270 bp

>T149614 NZ_CP064180:c4706051-4705665 [Citrobacter amalonaticus]
ATGCAAAAGGGACCCGTGTTGTTCGATACCAATATCCTGATTGATTTATTTAGCGGGCGCCAGGAAGCACAGCAAGTACT
GGAAGCGTATCCGCCGCAGAATGCCATCAGCTTGATTACCTGGATGGAAGTCATGGTGGGGGCAAAAAAATACCATCAGG
AATATCGTACGCGTGTGGCATTGAGTGCGTTCAATATCATCGGTGTATCACTGGAAATTGCTGAGCGAAGCGTGAATCTC
AGGCAGGAATATGGGATGAAACTTCCGGACGCGATCATTCTGGCTACCGCGCAGATTCATCGGTTTGCGCTTGTGACGCG
CAACACCAGAGATTTCGCGGGGATACCTGGCGTCATTACGCCTTATCAGTTGCAGGCCGGACGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8937.97 Da
• Isoelectric Point: 4.7254

>AT149614 WP_010971060.1 NZ_CP048558:c324726-324499 [Agrobacterium fabrum]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRRIQRGESKIISAEEFWRDLDN

Nucleotide

• Length: 228 bp

>AT149614 NZ_CP064180:c4706293-4706051 [Citrobacter amalonaticus]
ATAATGGCTGGAATGGACATGGGCAGAATATTACTCGACTTATCCGATGAGGTGATCAAACGCCTTGACGATCTCAAGCA
GCAGCGGAATCTGCCCCGTGCGGAGTTGCTGCGCGAAGCGGTTGAACAGTACCTTGAAAGGCAAGGCCAGGCTGAAACCA
CAATTTCCAATGCGCTGGGGCTCTGGCAGGGTTGTGAGGAAGACGGCGTTGAGTATCAGAGGACGCTGCGCGAGGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: A9CK21