Detailed information of TA system TA149613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4838..5531
• Replicon: plasmid pAt4
• Accession: NZ_CP048557
• Organism: Agrobacterium tumefaciens strain Kin002

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: FY136_RS29205
• Protein ID: WP_209160465.1
• Coordinates: 4838..5215 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: FY136_RS29210
• Protein ID: WP_262518623.1
• Coordinates: 5196..5531 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY136_RS29175 (FY136_29015)	855..1568	+	714	WP_262518618.1	hypothetical protein	-
FY136_RS29180 (FY136_29020)	1658..1867	+	210	WP_125302707.1	ribbon-helix-helix protein, CopG family	-
FY136_RS29185 (FY136_29025)	2053..2763	+	711	WP_262518619.1	helix-turn-helix domain-containing protein	-
FY136_RS29190 (FY136_29030)	3012..3665	+	654	WP_262518620.1	SprT-like domain-containing protein	-
FY136_RS29195 (FY136_29035)	3744..4403	+	660	WP_262518621.1	AAA family ATPase	-
FY136_RS29200 (FY136_29040)	4400..4741	+	342	WP_262518622.1	chromosome partitioning protein ParB	-
FY136_RS29205 (FY136_29045)	4838..5215	+	378	WP_209160465.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY136_RS29210 (FY136_29050)	5196..5531	+	336	WP_262518623.1	helix-turn-helix transcriptional regulator	Antitoxin
FY136_RS29215 (FY136_29055)	5648..7384	-	1737	WP_262518624.1	type IV secretion system DNA-binding domain-containing protein	-
FY136_RS29220 (FY136_29060)	7621..7896	-	276	WP_262518625.1	hypothetical protein	-
FY136_RS29225 (FY136_29065)	7898..9697	+	1800	WP_262518626.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..16069	16069

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13991.06 Da
• Isoelectric Point: 9.4141

>T149613 WP_209160465.1 NZ_CP048557:4838-5215 [Agrobacterium tumefaciens]
MKQEERPLEWIGSSYKDLMQLPADVRKSVGFALHFAQHGEQHPASKVLKGFGGAGVLEVVENDVGGTYRAVYTVRYEEAV
FVLHCFQKKSKSGIATPKEDMDVIRARLKVAEAIAKEMRDANARR

Nucleotide

• Length: 378 bp

>T149613 NZ_CP064180:c4381292-4380816 [Citrobacter amalonaticus]
ATGGATTTTCCCACATGGCTGAACGGGTGGACGATCTACGCTCATCCCTGTTTTATCGATCAATACAACGCGCTGGTCGC
ACGTGTTGAAGACCTTAAGCACAGGTTTCCAGATCCCATTGTTTTCCAGAAGAAGAAAGAGACCAAGGTGCTGGCGCATT
TATTGAAATCCATTGCCAACATTACCCACGAACCCCGAGCTTCTGTTTACCGGCCTGGTGACAGTATAGGGAAAGCTTAT
ACAGACTGGTCGAGAGCGAAGTTTGGTGGAGGACGGTATCGCTTATTTTTCCGTTATAGTCTCGAGAGTAAGATCATTGT
CATCGCATGGGTGAATGATGAAGGGCCATTGCGCACCTACGGCAGCAAGACCGATGCCTATAAGATTTTCGGGAAAATGC
TTGATGAGGGGAATCCACCCGACGACTGGCTAAGTTTGCTTCAGGCTTGTCAAAACGACGGGAAGGAACATCTTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12028.97 Da
• Isoelectric Point: 9.8605

>AT149613 WP_262518623.1 NZ_CP048557:5196-5531 [Agrobacterium tumefaciens]
MQTRVVNGIEVTTSSGNVFADLGLPDADKLKVKSGLMIEITKAVRRLGLTQEEAGRRMGLPQPKVSALLRGDFTNLSERK
LMDALNRLGYDIEITVRPTADPVGHLTLAVA

Nucleotide

• Length: 336 bp

>AT149613 NZ_CP064180:c4381632-4381297 [Citrobacter amalonaticus]
ATGTTCGCCTATGTTCGATCTGAAGACATTGCCATTGAAGCGGATTCTAAATTGACGGAACGACATCAAACGACTATCCC
TGCGTCGATCAGGGACGCTTTACATCTTCGCAGTGGTGATCGTATTCATTACACATTGCTTTCCACGGGTGAAGTACTGC
TTTCAAAATACAGTGAAGCAACGGAGGACGGTGTGATGCGATCATTCCTGGACTTCTTATCACAGGATGTGGCGAATAAC
CCACAAACGCTGAAGAAGCTGGATTTATCCCGTGGCTATGAACTGGTTGATGGTATCGACGTCAATCTTGATGATGAAAT
CAGCGACGAAGACTGA