Detailed information of TA system TA149609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 2191326..2191932
• Replicon: chromosome
• Accession: NZ_CP048551
• Organism: Agrobacterium tumefaciens strain Kin002

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: FY136_RS10965
• Protein ID: WP_142857388.1
• Coordinates: 2191326..2191622 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: FY136_RS10970
• Protein ID: WP_026363923.1
• Coordinates: 2191627..2191932 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY136_RS10940 (FY136_10865)	2187113..2188006	-	894	WP_262515924.1	hemin ABC transporter substrate-binding protein	-
FY136_RS10945 (FY136_10870)	2188203..2188550	+	348	WP_065114087.1	antibiotic biosynthesis monooxygenase	-
FY136_RS10950 (FY136_10875)	2188559..2189080	+	522	WP_262515925.1	heme utilization cystosolic carrier protein HutX	-
FY136_RS10955 (FY136_10880)	2189412..2190188	+	777	WP_262515927.1	YbaY family lipoprotein	-
FY136_RS10960 (FY136_10885)	2190396..2191280	+	885	WP_206913426.1	formyltetrahydrofolate deformylase	-
FY136_RS10965 (FY136_10890)	2191326..2191622	+	297	WP_142857388.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY136_RS10970 (FY136_10895)	2191627..2191932	+	306	WP_026363923.1	putative addiction module antidote protein	Antitoxin
FY136_RS10975 (FY136_10900)	2191945..2193339	-	1395	WP_262515930.1	sensor histidine kinase	-
FY136_RS10980 (FY136_10905)	2193336..2194016	-	681	WP_262515931.1	response regulator transcription factor	-
FY136_RS10985 (FY136_10910)	2194098..2195165	+	1068	WP_065116048.1	ABC transporter substrate-binding protein	-
FY136_RS10990 (FY136_10915)	2195508..2196452	+	945	WP_262515933.1	tripartite tricarboxylate transporter substrate binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (6-91)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10892.60 Da
• Isoelectric Point: 10.2733

>T149609 WP_142857388.1 NZ_CP048551:2191326-2191622 [Agrobacterium tumefaciens]
MLTIRETSEFADWLAGLRDLQARARIARRIYRLADGNPGDVKPVGEGVSELRIDLGPGYRVYFVRHGDVVIILLCGGDKA
SQSRDIARAKKLARALKE

Nucleotide

• Length: 297 bp

>T149609 NZ_CP064180:1852578-1852835 [Citrobacter amalonaticus]
ATGAATTTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCCAAAGGTGGACGTATTGCGCACGTCCGCGATGCGTC
AGGCCGCGTCACCGCCGTTGAATGCTACAGCCGCGAAGGGCTGCTGCTCGCCGACTGTACACTTGCCGTCTTCAAAAAAC
TCAAAACCAAAAAGCTGATCAAATCCGTCAATGGTCAGCCGTACCGCATTAATACCACCGGGCTGAACAACGTGCGTGCT
CAGCCAGATAACCGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10566.86 Da
• Isoelectric Point: 4.5580

>AT149609 WP_026363923.1 NZ_CP048551:2191627-2191932 [Agrobacterium tumefaciens]
MPLVTRDYDAARYLDSDEALAAYIADATESGDAQELAHALGVVARAKGMTDLSRQTGLPRQTLYKALSGEGNPELATIAK
VADALGYRLSLVAKSPADTAA

Nucleotide

• Length: 306 bp

>AT149609 NZ_CP064180:1852846-1853397 [Citrobacter amalonaticus]
ATGACAACACGCAACTTCACTATTCATATTACCGATGAAAGTGATACCAACGACATCCGGGAAGTCGAAACTCGCGCCTT
TGGCTACAGTAAAGAGGCCTGCCTGGTGGCTTCTTTGCTCGAGGACGACACTGCGCGTCCAACGTTATCGTTGCTGGCCA
GACAGGAAGGTAACGCCGTCGGGCATATCCTGTTTACCCGGGCGACATTCAAAGGAGAAATGGATTCCCCTCTGATGCAT
ATCCTTGCTCCCCTGGCGGTCATCCCTGAATACCAGGGGAGGGGCGTGGGCGGATTATTGATTCGTACCGGTATAGAACA
CTTACGCTCGATGGGATGCCAGTGCGTTTTTGTTCTCGGTCATGCCACTTACTATCCGCGACATGGATTTGAACCCTGTG
CAGGAGACAAAGGCTTTCCCGCGCCTTACCCCATTGCGGAGGAACATAAGGCCTGCTGGATGATGCAGTCCCTCTCCGCA
CAACCCTTTGATCGTGCCGGCGACATCCAATGTGCGCAGGCCTTAATGAAACCGGAGCACTGGCGTGAATAA