Detailed information of TA system TA149604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 496770..497314
• Replicon: chromosome
• Accession: NZ_CP048543
• Organism: Agrobacterium tumefaciens strain SJ001

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: FY138_RS02490
• Protein ID: WP_173992172.1
• Coordinates: 497024..497314 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A822V4A8
• Locus tag: FY138_RS02485
• Protein ID: WP_003509084.1
• Coordinates: 496770..497021 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY138_RS02465 (FY138_02455)	492531..493211	+	681	WP_003509096.1	GntR family transcriptional regulator	-
FY138_RS02470 (FY138_02460)	493257..493733	+	477	WP_099085593.1	DUF1284 domain-containing protein	-
FY138_RS02475 (FY138_02465)	493909..495009	-	1101	WP_099085592.1	hypothetical protein	-
FY138_RS02480 (FY138_02470)	495246..496391	+	1146	WP_087743499.1	site-specific DNA-methyltransferase	-
FY138_RS02485 (FY138_02475)	496770..497021	+	252	WP_003509084.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
FY138_RS02490 (FY138_02480)	497024..497314	+	291	WP_173992172.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY138_RS02495 (FY138_02485)	497356..497967	-	612	WP_087743505.1	HAD family phosphatase	-
FY138_RS02500 (FY138_02490)	497964..499076	-	1113	WP_099085591.1	A/G-specific adenine glycosylase	-
FY138_RS02505 (FY138_02495)	499204..499668	+	465	WP_020812858.1	hypothetical protein	-
FY138_RS02510 (FY138_02500)	499696..500217	+	522	WP_003509079.1	DUF721 domain-containing protein	-
FY138_RS02515 (FY138_02505)	500346..501026	+	681	WP_003509078.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (12-70)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11225.88 Da
• Isoelectric Point: 6.2182

>T149604 WP_173992172.1 NZ_CP048543:497024-497314 [Agrobacterium tumefaciens]
VGFRLSLLAEEDVIRIAEEGIAIFGLAQAQKYHRELYEIFELISLNPRMARERHELLPPLRIHRFRTHLVVYGVGADDDV
LIVRVRHAHEDWVNES

Nucleotide

• Length: 291 bp

>T149604 NZ_CP064174:c4868819-4868451 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9339.55 Da
• Isoelectric Point: 9.7317

>AT149604 WP_003509084.1 NZ_CP048543:496770-497021 [Agrobacterium tumefaciens]
MATMNISLPAPMKEWVEAQSRTGRYSNASDYVRDLIRKDQERGEKIAAMQHFVDEGLQSGAGHRSQQALFAEAEARSMKR
KIG

Nucleotide

• Length: 252 bp

>AT149604 NZ_CP064174:c4869037-4868816 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A822V4A8