Detailed information of TA system TA149598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 332716..333196
• Replicon: chromosome
• Accession: NZ_CP048537
• Organism: Agrobacterium tumefaciens strain SJ003

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A437L1W4
• Locus tag: FY140_RS11885
• Protein ID: WP_003519526.1
• Coordinates: 332716..332985 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: F0L6V5
• Locus tag: FY140_RS11890
• Protein ID: WP_013635548.1
• Coordinates: 332969..333196 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY140_RS11850 (FY140_11780)	327898..328527	+	630	WP_262534848.1	DNA-3-methyladenine glycosylase I	-
FY140_RS11855 (FY140_11785)	328524..328967	+	444	WP_262534849.1	hypothetical protein	-
FY140_RS11860 (FY140_11790)	328983..330026	-	1044	WP_120947604.1	YeiH family protein	-
FY140_RS11865 (FY140_11795)	330108..331025	+	918	WP_020812649.1	LysR substrate-binding domain-containing protein	-
FY140_RS11870 (FY140_11800)	331073..331447	-	375	WP_262534850.1	DUF305 domain-containing protein	-
FY140_RS11875 (FY140_11805)	331505..331807	-	303	WP_262534851.1	hypothetical protein	-
FY140_RS11880 (FY140_11810)	331945..332709	-	765	WP_137002952.1	hypothetical protein	-
FY140_RS11885 (FY140_11815)	332716..332985	-	270	WP_003519526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY140_RS11890 (FY140_11820)	332969..333196	-	228	WP_013635548.1	DUF6290 family protein	Antitoxin
FY140_RS11895 (FY140_11825)	333354..334877	+	1524	WP_262534852.1	histidine--tRNA ligase	-
FY140_RS11900 (FY140_11830)	334882..335250	+	369	WP_262534853.1	VOC family protein	-
FY140_RS11905 (FY140_11835)	335267..336394	+	1128	WP_137452312.1	ATP phosphoribosyltransferase regulatory subunit	-
FY140_RS11910 (FY140_11840)	336391..337083	+	693	WP_080817660.1	ATP phosphoribosyltransferase	-
FY140_RS11915 (FY140_11845)	337146..337592	-	447	WP_127756301.1	DoxX family protein	-
FY140_RS11920 (FY140_11850)	337744..338178	-	435	WP_262534854.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (6-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10687.30 Da
• Isoelectric Point: 9.5690

>T149598 WP_003519526.1 NZ_CP048537:c332985-332716 [Agrobacterium tumefaciens]
VIWTIEYHTLVQKEMRKINPETRQRIRSFLHERLAALDDPRQTGAALQGSELGNFWRYRVGDYRIICDIQDHKLVVLVVE
IGHRREIYR

Nucleotide

• Length: 270 bp

>T149598 NZ_CP064174:c1185013-1184795 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8909.92 Da
• Isoelectric Point: 4.7254

>AT149598 WP_013635548.1 NZ_CP048537:c333196-332969 [Agrobacterium tumefaciens]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRRIQRGESKVVSAEEFWRDLDN

Nucleotide

• Length: 228 bp

>AT149598 NZ_CP064174:c1185414-1185040 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A437L1W4

Antitoxin

• AlphaFold DB: F0L6V5