Detailed information of TA system TA149596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 686004..686584
• Replicon: chromosome
• Accession: NZ_CP048536
• Organism: Agrobacterium tumefaciens strain SJ003

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: FY140_RS02985
• Protein ID: WP_262531336.1
• Coordinates: 686004..686270 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A420FEH2
• Locus tag: FY140_RS02990
• Protein ID: WP_059753616.1
• Coordinates: 686261..686584 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY140_RS02960 (FY140_02945)	682479..682994	+	516	WP_262531333.1	disulfide bond formation protein B	-
FY140_RS02965 (FY140_02950)	683025..683582	-	558	WP_003585610.1	HNH endonuclease	-
FY140_RS02970 (FY140_02955)	683930..684229	+	300	WP_262531334.1	hypothetical protein	-
FY140_RS02975 (FY140_02960)	684377..684982	+	606	WP_080819306.1	transglutaminase-like cysteine peptidase	-
FY140_RS02980 (FY140_02965)	685178..685969	+	792	WP_262531335.1	metallophosphoesterase family protein	-
FY140_RS02985 (FY140_02970)	686004..686270	+	267	WP_262531336.1	BrnT family toxin	Toxin
FY140_RS02990 (FY140_02975)	686261..686584	+	324	WP_059753616.1	BrnA antitoxin family protein	Antitoxin
FY140_RS02995 (FY140_02980)	686657..687883	-	1227	WP_262531337.1	glycosyltransferase family 4 protein	-
FY140_RS03000 (FY140_02985)	687880..689217	-	1338	WP_262531338.1	polysaccharide pyruvyl transferase family protein	-
FY140_RS03005 (FY140_02990)	689210..690688	-	1479	WP_080819311.1	lipopolysaccharide biosynthesis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (16-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 9797.61 Da
• Isoelectric Point: 7.7178

>T149596 WP_262531336.1 NZ_CP048536:686004-686270 [Agrobacterium tumefaciens]
MPLIYCTYIICKSLMTNIEKHGLDFADLTIEFFAGSIVLPAKEDRFMAIGEFQGRIIIAVVFSPLGSQALSVISMRPAST
RERRLSCP

Nucleotide

• Length: 267 bp

>T149596 NZ_CP064174:470348-470632 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11898.70 Da
• Isoelectric Point: 10.1991

>AT149596 WP_059753616.1 NZ_CP048536:686261-686584 [Agrobacterium tumefaciens]
MPVKFSSKRPLTNKEEAEIQKMIASDPDAPEATDKEMAQAKPFGAVFPDLAKSIDREIARRGRPKADAPKTPVTIRLDPD
LVEHYKAMGKGWQSRINSDLRKLSGLP

Nucleotide

• Length: 324 bp

>AT149596 NZ_CP064174:470116-470358 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A420FEH2