Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 372038..372669 Replicon plasmid pAt
Accession NZ_CP048520
Organism Agrobacterium tumefaciens strain Sta005

Toxin (Protein)


Gene name vapC Uniprot ID A0A822VAP2
Locus tag FY144_RS25590 Protein ID WP_060725879.1
Coordinates 372038..372439 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A822VB92
Locus tag FY144_RS25595 Protein ID WP_060725878.1
Coordinates 372439..372669 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FY144_RS25565 (FY144_25375) 367542..368330 - 789 WP_121593584.1 SDR family oxidoreductase -
FY144_RS25570 (FY144_25380) 368440..369000 + 561 WP_162993134.1 TetR/AcrR family transcriptional regulator -
FY144_RS25575 (FY144_25385) 369909..370739 + 831 WP_121593586.1 AraC family transcriptional regulator -
FY144_RS25580 (FY144_25390) 370851..371495 + 645 WP_060725880.1 LysE family translocator -
FY144_RS25585 (FY144_25395) 371732..371962 + 231 WP_060726006.1 BrnA antitoxin family protein -
FY144_RS25590 (FY144_25400) 372038..372439 - 402 WP_060725879.1 tRNA(fMet)-specific endonuclease VapC Toxin
FY144_RS25595 (FY144_25405) 372439..372669 - 231 WP_060725878.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FY144_RS25600 (FY144_25410) 372881..374512 - 1632 WP_121593587.1 GMC family oxidoreductase -
FY144_RS25605 (FY144_25415) 374705..375499 - 795 WP_121593588.1 alpha/beta hydrolase -
FY144_RS25610 (FY144_25420) 375644..376111 + 468 WP_121593589.1 MarR family transcriptional regulator -
FY144_RS25615 (FY144_25425) 376138..376917 - 780 WP_121593590.1 SDR family oxidoreductase -
FY144_RS25620 (FY144_25430) 376914..377243 - 330 WP_121593591.1 nuclear transport factor 2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB 1..520191 520191


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-124)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14936.03 Da        Isoelectric Point: 7.2437

>T149589 WP_060725879.1 NZ_CP048520:c372439-372038 [Agrobacterium tumefaciens]
MLKYMLDTNICIFTIKNRPQQVRDAFNRSHDQLCISSVSLMELIYGAEKSASPEKNLSVVEGFAARLEVLPYDELAASHT
GQLRAELARSGTPIGPYDQLIAGHARSRGLVMVTNNRREFDRLPGLRVEDWTS

Download         Length: 402 bp

>T149589 NZ_CP064168:c3127-2087 [Escherichia coli O157:H7]
ATGGCACAGAACCCGTTTAAAGCACTAAATATCAATATTGACAAGATTGAGTCTGCTCTGACGCAGAATGGCGTCACAAA
CTATTCCTCTAATGTAAAAAACGAAAGAGAAACTCACATATCTGGCACATATAAAGGAATAGACTTCTTAATAAAACTAA
TGCCATCAGGCGGAAATACCACTATCGGAAGAGCGTCTGGGCAAAATAACACTTACTTTGATGAAATCGCCTTGATTATA
AAAGAAAACTGTTTGTATTCAGACACAAAGAACTTTGAATACACCATTCCAAAATTCAGTGATGATGACAGGGCAAATCT
ATTTGAATTTCTTTCTGAAGAGGGGATAACAATAACAGAAGATAATAATAACGATCCTAATTGTAAACACCAATATATTA
TGACCACCAGCAATGGTGACAGGGTCAGGGCAAAAATTTACAAGCGCGGCTCTATTCAATTCCAAGGAAAATACCTTCAA
ATCGCGAGTTTGATTAACGATTTCATGTGCTCAATACTAAACATGAAAGAGATTGTCGAACAAAAAAATAAAGAATTTAA
TGTTGACATAAAAAAAGAAACTATTGAGTCCGAGTTGCATAGCAAACTACCAAAAAGCATCGATAAAATCCATGAAGATA
TCAAAAAACAGCTATCATGCTCGCTAATAATGAAAAAAATAGATGTCGAAATGGAAGATTACTCAACATACTGCTTCTCT
GCATTAAGAGCCATAGAAGGCTTTATATATCAAATACTTAATGATGTTTGCAATCCATCATCATCAAAGAACCTTGGCGA
ATACTTCACTGAAAACAAACCCAAATATATAATCAGAGAAATACACCAAGAAACTATAAATGGTGAAATAGCGGAAGTTT
TGTGTGAATGCTACACTTACTGGCATGAGAACAGGCATGGTTTGTTTCATATGAAACCAGGAATAGCTGACACGAAGACA
ATTAACAAATTAGAATCAATCGCAATCATCGATACCGTTTGCCAATTAATAGATGGTGGCGTAGCTAGGTTGAAATTATG
A

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8474.53 Da        Isoelectric Point: 4.3719

>AT149589 WP_060725878.1 NZ_CP048520:c372669-372439 [Agrobacterium tumefaciens]
MEQGAVFQSNRSQAIRLPKAVALPDDVKRVDIVAVGRTRIIAPAGEIWDSWFEGAEVTSDFMIERDQAAPQERDAF

Download         Length: 231 bp

>AT149589 NZ_CP064168:c2090-1710 [Escherichia coli O157:H7]
ATGAAAAAAGATAAAAAATATCAAATAGAAGCAATAAAAAATAAAGATAAAACTTTATTTATTGTCTATGCTACTGATAT
TTATAGCCCGAGCGAATTTTTCTCAAAAATCGAATCCGACTTGAAGAAAAAGAAAAGCAAGGGTGATGTTTTTTTTGATT
TAATAATTCCTAACGGTGGAAAAAAAGATCGTTACGTCTATACGTCATTTAATGGCGAGAAGTTTTCAAGTTACACATTA
AACAAAGTTACGAAAACTGATGAATATAATGATTTATCTGAGCTCTCGGCTTCGTTCTTTAAAAAAAACTTTGATAAGAT
CAACGTAAACCTTCTATCCAAAGCCACATCATTTGCTTTGAAAAAAGGCATTCCAATATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822VAP2

Antitoxin

Source ID Structure
AlphaFold DB A0A822VB92

References