Detailed information of TA system TA149588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 142359..143029
• Replicon: plasmid pAt
• Accession: NZ_CP048520
• Organism: Agrobacterium tumefaciens strain Sta005

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FY144_RS24310
• Protein ID: WP_121593481.1
• Coordinates: 142610..143029 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A822VAD5
• Locus tag: FY144_RS24305
• Protein ID: WP_060726010.1
• Coordinates: 142359..142613 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY144_RS24285 (FY144_24100)	137571..139163	-	1593	WP_121593480.1	ABC transporter substrate-binding protein	-
FY144_RS24290 (FY144_24105)	139279..139989	+	711	WP_112960072.1	GntR family transcriptional regulator	-
FY144_RS24295 (FY144_24110)	139991..140911	+	921	WP_112960071.1	dihydrodipicolinate synthase family protein	-
FY144_RS24300 (FY144_24115)	140925..142064	+	1140	WP_112960070.1	mandelate racemase/muconate lactonizing enzyme family protein	-
FY144_RS24305 (FY144_24120)	142359..142613	+	255	WP_060726010.1	hypothetical protein	Antitoxin
FY144_RS24310 (FY144_24125)	142610..143029	+	420	WP_121593481.1	type II toxin-antitoxin system VapC family toxin	Toxin
FY144_RS24315 (FY144_24130)	143119..143871	-	753	WP_060725901.1	FadR/GntR family transcriptional regulator	-
FY144_RS24320 (FY144_24135)	144329..144802	+	474	WP_060725902.1	RidA family protein	-
FY144_RS24325 (FY144_24140)	144821..145576	+	756	WP_174014749.1	amino acid ABC transporter ATP-binding protein	-
FY144_RS24330 (FY144_24145)	145587..146255	+	669	WP_060725903.1	amino acid ABC transporter permease	-
FY144_RS24335 (FY144_24150)	146265..146915	+	651	WP_060725904.1	amino acid ABC transporter permease	-
FY144_RS24340 (FY144_24155)	146955..147800	+	846	WP_121593482.1	transporter substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..520191	520191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15208.56 Da
• Isoelectric Point: 5.1409

>T149588 WP_121593481.1 NZ_CP048520:142610-143029 [Agrobacterium tumefaciens]
MILLDTNVISEPWKPVPDEAVIAWLDAQVLETLFISSITIAELRFGIAAMPFGKRQTTLRDRLEGEVLPHFSRRILFFDL
ATSQFYSDLIARARLSGKAIGTTDGYIAATAAANGLAIATRDTSPFEAAGLKVINPWSI

Nucleotide

• Length: 420 bp

>T149588 NZ_CP064167:5383276-5383650 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9333.53 Da
• Isoelectric Point: 6.2300

>AT149588 WP_060726010.1 NZ_CP048520:142359-142613 [Agrobacterium tumefaciens]
MPAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDIIEAAVRPPERIKLGTLLTGIGRDAELSESDVEALQKSRDKTTAEP
MTFE

Nucleotide

• Length: 255 bp

>AT149588 NZ_CP064167:5382855-5383229 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A822VAD5