Detailed information of TA system TA149587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 2115889..2116495
• Replicon: chromosome
• Accession: NZ_CP048518
• Organism: Agrobacterium tumefaciens strain Sta005

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A822UZY8
• Locus tag: FY144_RS10655
• Protein ID: WP_020011654.1
• Coordinates: 2115889..2116185 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A822V132
• Locus tag: FY144_RS10660
• Protein ID: WP_060723677.1
• Coordinates: 2116190..2116495 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY144_RS10620 (FY144_10535)	2111483..2111830	+	348	WP_020809369.1	antibiotic biosynthesis monooxygenase	-
FY144_RS10625 (FY144_10540)	2111840..2112364	+	525	WP_060723671.1	heme utilization cystosolic carrier protein HutX	-
FY144_RS10630 (FY144_10545)	2112549..2112938	+	390	WP_233282764.1	hypothetical protein	-
FY144_RS10635 (FY144_10550)	2113091..2113630	+	540	WP_060723673.1	DUF2975 domain-containing protein	-
FY144_RS10640 (FY144_10555)	2113640..2113882	+	243	WP_060723674.1	helix-turn-helix transcriptional regulator	-
FY144_RS10645 (FY144_10560)	2113976..2114752	+	777	WP_060723675.1	YbaY family lipoprotein	-
FY144_RS10650 (FY144_10565)	2114959..2115843	+	885	WP_060723676.1	formyltetrahydrofolate deformylase	-
FY144_RS10655 (FY144_10570)	2115889..2116185	+	297	WP_020011654.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY144_RS10660 (FY144_10575)	2116190..2116495	+	306	WP_060723677.1	putative addiction module antidote protein	Antitoxin
FY144_RS10665 (FY144_10580)	2116509..2117903	-	1395	WP_065695049.1	sensor histidine kinase	-
FY144_RS10670 (FY144_10585)	2117900..2118580	-	681	WP_003491888.1	response regulator transcription factor	-
FY144_RS10675 (FY144_10590)	2118662..2119729	+	1068	WP_060723730.1	ABC transporter substrate-binding protein	-
FY144_RS10680 (FY144_10595)	2119963..2120892	+	930	WP_121592187.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10888.61 Da
• Isoelectric Point: 10.2733

>T149587 WP_020011654.1 NZ_CP048518:2115889-2116185 [Agrobacterium tumefaciens]
MLTIRETSEFADWLAGLRDVQARARIARRIYRLADGNPGDVKPVGEGVSELRIDLGPGYRVYFVRHGDVVIILLCGGDKA
SQPRDIARAKKLARALKE

Nucleotide

• Length: 297 bp

>T149587 NZ_CP064167:4176932-4177267 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10576.90 Da
• Isoelectric Point: 4.5580

>AT149587 WP_060723677.1 NZ_CP048518:2116190-2116495 [Agrobacterium tumefaciens]
MPLVTRDYDAARYLDSDEALAAYIADATESGDAQELAHALGVVARAKGMTDLSRQTGLPRQTLYKALSGEGNPELATIAK
VADALGYRLSLVAKPPADTAA

Nucleotide

• Length: 306 bp

>AT149587 NZ_CP064167:4176684-4176932 [Escherichia coli O157:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A822UZY8

Antitoxin

• AlphaFold DB: A0A822V132