Detailed information of TA system TA149585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 451917..452461
• Replicon: chromosome
• Accession: NZ_CP048518
• Organism: Agrobacterium tumefaciens strain Sta005

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: FY144_RS02195
• Protein ID: WP_026363704.1
• Coordinates: 452171..452461 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A822V4A8
• Locus tag: FY144_RS02190
• Protein ID: WP_003509084.1
• Coordinates: 451917..452168 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY144_RS02170 (FY144_02170)	447679..448359	+	681	WP_003509096.1	GntR family transcriptional regulator	-
FY144_RS02175 (FY144_02175)	448405..448881	+	477	WP_060723039.1	DUF1284 domain-containing protein	-
FY144_RS02180 (FY144_02180)	449057..450157	-	1101	WP_111798193.1	hypothetical protein	-
FY144_RS02185 (FY144_02185)	450394..451539	+	1146	WP_020010628.1	site-specific DNA-methyltransferase	-
FY144_RS02190 (FY144_02190)	451917..452168	+	252	WP_003509084.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
FY144_RS02195 (FY144_02195)	452171..452461	+	291	WP_026363704.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY144_RS02200 (FY144_02200)	452503..453114	-	612	WP_003509082.1	HAD family phosphatase	-
FY144_RS02205 (FY144_02205)	453111..454223	-	1113	WP_020812857.1	A/G-specific adenine glycosylase	-
FY144_RS02210 (FY144_02210)	454351..454815	+	465	WP_020812858.1	hypothetical protein	-
FY144_RS02215 (FY144_02215)	454843..455364	+	522	WP_003509079.1	DUF721 domain-containing protein	-
FY144_RS02220 (FY144_02220)	455493..456173	+	681	WP_003509078.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (12-70)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11195.86 Da
• Isoelectric Point: 6.2182

>T149585 WP_026363704.1 NZ_CP048518:452171-452461 [Agrobacterium tumefaciens]
VGFRLSLLAEEDVIRIAEEGIAIFGLAQAQKYHRELYEIFELISLNPRMARERHELLPPLRIHRFRAHLVVYGVGADDDV
LIVRVRHAHEDWVNES

Nucleotide

• Length: 291 bp

>T149585 NZ_CP064167:3836335-3836649 [Escherichia coli O157:H7]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9339.55 Da
• Isoelectric Point: 9.7317

>AT149585 WP_003509084.1 NZ_CP048518:451917-452168 [Agrobacterium tumefaciens]
MATMNISLPAPMKEWVEAQSRTGRYSNASDYVRDLIRKDQERGEKIAAMQHFVDEGLQSGAGHRSQQALFAEAEARSMKR
KIG

Nucleotide

• Length: 252 bp

>AT149585 NZ_CP064167:3836646-3837062 [Escherichia coli O157:H7]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A822V4A8