Detailed information of TA system TA149583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 540929..541523
• Replicon: chromosome
• Accession: NZ_CP048513
• Organism: Agrobacterium tumefaciens strain Sut001

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: FY145_RS02705
• Protein ID: WP_080865974.1
• Coordinates: 541209..541523 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FY145_RS02700
• Protein ID: WP_080865973.1
• Coordinates: 540929..541228 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY145_RS02675 (FY145_02670)	536117..537115	+	999	WP_060723902.1	asparaginase	-
FY145_RS02680 (FY145_02675)	537250..537870	+	621	WP_003508980.1	glutathione S-transferase family protein	-
FY145_RS02685 (FY145_02680)	537964..538623	-	660	WP_003508979.1	glutathione S-transferase family protein	-
FY145_RS02690 (FY145_02685)	538706..539221	-	516	WP_099085580.1	MarR family transcriptional regulator	-
FY145_RS02695 (FY145_02690)	539363..540892	+	1530	WP_060723904.1	MDR family MFS transporter	-
FY145_RS02700 (FY145_02695)	540929..541228	-	300	WP_080865973.1	putative addiction module antidote protein	Antitoxin
FY145_RS02705 (FY145_02700)	541209..541523	-	315	WP_080865974.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY145_RS02710 (FY145_02705)	541754..542770	+	1017	WP_163117905.1	ligase-associated DNA damage response exonuclease	-
FY145_RS02715 (FY145_02710)	542772..544397	+	1626	WP_099085579.1	cisplatin damage response ATP-dependent DNA ligase	-
FY145_RS02720 (FY145_02715)	544572..545537	+	966	WP_020813204.1	alpha/beta fold hydrolase	-
FY145_RS02725 (FY145_02720)	545576..546358	-	783	WP_003508963.1	glycosyltransferase family 32 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-95)

Antitoxin

• Uncharacterized protein HI1420 (6-97)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11772.48 Da
• Isoelectric Point: 10.3857

>T149583 WP_080865974.1 NZ_CP048513:c541523-541209 [Agrobacterium tumefaciens]
MNSFQKSAEFDEWLKVLKDRAGKVRILHRLTAAEHGNFGDCAPVGEGVSEMRIHFGPGYRVYFTRVGKRVYFLLVGGDKS
SQRRDIKRAIEMAQTIGRDGRDGI

Nucleotide

• Length: 315 bp

>T149583 NZ_CP064167:c3502897-3502412 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10765.29 Da
• Isoelectric Point: 4.8307

>AT149583 WP_080865973.1 NZ_CP048513:c541228-540929 [Agrobacterium tumefaciens]
MAVTEFSKFDVSDYLDSDEAMIEFLAIAMEDENPDVFIGALGAVAKARGMSHIADASGLGRESLYKALRPGSKLRYETVQ
RVLHALGFRLAVEKSLNAA

Nucleotide

• Length: 300 bp

>AT149583 NZ_CP064167:c3503166-3502888 [Escherichia coli O157:H7]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA