Detailed information of TA system TA149580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 496959..497503
• Replicon: chromosome
• Accession: NZ_CP048508
• Organism: Agrobacterium tumefaciens strain Sut002

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: FY146_RS02500
• Protein ID: WP_173992172.1
• Coordinates: 497213..497503 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A822V4A8
• Locus tag: FY146_RS02495
• Protein ID: WP_003509084.1
• Coordinates: 496959..497210 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY146_RS02475 (FY146_02470)	492720..493400	+	681	WP_003509096.1	GntR family transcriptional regulator	-
FY146_RS02480 (FY146_02475)	493446..493922	+	477	WP_099085593.1	DUF1284 domain-containing protein	-
FY146_RS02485 (FY146_02480)	494098..495198	-	1101	WP_099085592.1	hypothetical protein	-
FY146_RS02490 (FY146_02485)	495435..496580	+	1146	WP_087743499.1	site-specific DNA-methyltransferase	-
FY146_RS02495 (FY146_02490)	496959..497210	+	252	WP_003509084.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
FY146_RS02500 (FY146_02495)	497213..497503	+	291	WP_173992172.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY146_RS02505 (FY146_02500)	497545..498156	-	612	WP_087743505.1	HAD family phosphatase	-
FY146_RS02510 (FY146_02505)	498153..499265	-	1113	WP_099085591.1	A/G-specific adenine glycosylase	-
FY146_RS02515 (FY146_02510)	499393..499857	+	465	WP_020812858.1	hypothetical protein	-
FY146_RS02520 (FY146_02515)	499885..500406	+	522	WP_003509079.1	DUF721 domain-containing protein	-
FY146_RS02525 (FY146_02520)	500535..501215	+	681	WP_003509078.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (12-70)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11225.88 Da
• Isoelectric Point: 6.2182

>T149580 WP_173992172.1 NZ_CP048508:497213-497503 [Agrobacterium tumefaciens]
VGFRLSLLAEEDVIRIAEEGIAIFGLAQAQKYHRELYEIFELISLNPRMARERHELLPPLRIHRFRTHLVVYGVGADDDV
LIVRVRHAHEDWVNES

Nucleotide

• Length: 291 bp

>T149580 NZ_CP048508:497213-497503 [Agrobacterium tumefaciens]
GTGGGCTTTCGCCTGTCTCTGCTCGCGGAAGAAGACGTCATTCGAATAGCCGAAGAAGGCATCGCGATATTCGGGCTGGC
GCAGGCGCAAAAATATCACCGGGAACTCTATGAGATATTTGAGCTGATATCTCTCAACCCACGCATGGCACGCGAGCGGC
ACGAGTTGTTACCGCCGCTGCGTATCCATCGGTTCAGGACACACCTGGTTGTTTATGGCGTGGGAGCGGACGATGATGTC
TTGATCGTCCGCGTCCGTCACGCACATGAGGACTGGGTCAACGAGTCGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9339.55 Da
• Isoelectric Point: 9.7317

>AT149580 WP_003509084.1 NZ_CP048508:496959-497210 [Agrobacterium tumefaciens]
MATMNISLPAPMKEWVEAQSRTGRYSNASDYVRDLIRKDQERGEKIAAMQHFVDEGLQSGAGHRSQQALFAEAEARSMKR
KIG

Nucleotide

• Length: 252 bp

>AT149580 NZ_CP048508:496959-497210 [Agrobacterium tumefaciens]
ATGGCGACCATGAACATATCGCTGCCTGCACCCATGAAAGAATGGGTTGAGGCCCAATCCAGGACAGGCCGTTATTCGAA
TGCGAGCGACTACGTGCGAGACCTGATCCGCAAGGATCAGGAGCGAGGCGAAAAAATCGCGGCGATGCAGCATTTTGTGG
ATGAGGGATTGCAGAGCGGTGCCGGGCATCGCTCGCAACAGGCCTTGTTCGCCGAGGCTGAAGCTCGTTCCATGAAACGC
AAGATCGGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A822V4A8