Detailed information of TA system TA149578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 470936..471480
• Replicon: chromosome
• Accession: NZ_CP048502
• Organism: Agrobacterium tumefaciens strain Teh001

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: FY147_RS02255
• Protein ID: WP_173992172.1
• Coordinates: 471190..471480 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A822V4A8
• Locus tag: FY147_RS02250
• Protein ID: WP_003509084.1
• Coordinates: 470936..471187 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY147_RS02230 (FY147_02245)	466697..467377	+	681	WP_003509096.1	GntR family transcriptional regulator	-
FY147_RS02235 (FY147_02250)	467423..467899	+	477	WP_099085593.1	DUF1284 domain-containing protein	-
FY147_RS02240 (FY147_02255)	468075..469175	-	1101	WP_262513308.1	hypothetical protein	-
FY147_RS02245 (FY147_02260)	469412..470557	+	1146	WP_087743499.1	site-specific DNA-methyltransferase	-
FY147_RS02250 (FY147_02265)	470936..471187	+	252	WP_003509084.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
FY147_RS02255 (FY147_02270)	471190..471480	+	291	WP_173992172.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY147_RS02260 (FY147_02275)	471522..472133	-	612	WP_087743505.1	HAD family phosphatase	-
FY147_RS02265 (FY147_02280)	472130..473242	-	1113	WP_099085591.1	A/G-specific adenine glycosylase	-
FY147_RS02270 (FY147_02285)	473370..473834	+	465	WP_020812858.1	hypothetical protein	-
FY147_RS02275 (FY147_02290)	473862..474383	+	522	WP_003509079.1	DUF721 domain-containing protein	-
FY147_RS02280 (FY147_02295)	474512..475192	+	681	WP_003509078.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (12-70)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11225.88 Da
• Isoelectric Point: 6.2182

>T149578 WP_173992172.1 NZ_CP048502:471190-471480 [Agrobacterium tumefaciens]
VGFRLSLLAEEDVIRIAEEGIAIFGLAQAQKYHRELYEIFELISLNPRMARERHELLPPLRIHRFRTHLVVYGVGADDDV
LIVRVRHAHEDWVNES

Nucleotide

• Length: 291 bp

>T149578 NZ_CP064167:c2611235-2611062 [Escherichia coli O157:H7]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9339.55 Da
• Isoelectric Point: 9.7317

>AT149578 WP_003509084.1 NZ_CP048502:470936-471187 [Agrobacterium tumefaciens]
MATMNISLPAPMKEWVEAQSRTGRYSNASDYVRDLIRKDQERGEKIAAMQHFVDEGLQSGAGHRSQQALFAEAEARSMKR
KIG

Nucleotide

• Length: 252 bp

>AT149578 NZ_CP064167:c2611559-2611263 [Escherichia coli O157:H7]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A822V4A8