Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 504516..505110 Replicon chromosome
Accession NZ_CP048493
Organism Agrobacterium tumefaciens strain Tul001

Toxin (Protein)


Gene name PumA Uniprot ID -
Locus tag FY149_RS12775 Protein ID WP_080865974.1
Coordinates 504796..505110 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag FY149_RS12770 Protein ID WP_080865973.1
Coordinates 504516..504815 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FY149_RS12745 (FY149_12685) 499704..500702 + 999 WP_060723902.1 asparaginase -
FY149_RS12750 (FY149_12690) 500837..501457 + 621 WP_003508980.1 glutathione S-transferase family protein -
FY149_RS12755 (FY149_12695) 501551..502210 - 660 WP_003508979.1 glutathione S-transferase family protein -
FY149_RS12760 (FY149_12700) 502293..502808 - 516 WP_099085580.1 MarR family transcriptional regulator -
FY149_RS12765 (FY149_12705) 502950..504479 + 1530 WP_060723904.1 MDR family MFS transporter -
FY149_RS12770 (FY149_12710) 504516..504815 - 300 WP_080865973.1 putative addiction module antidote protein Antitoxin
FY149_RS12775 (FY149_12715) 504796..505110 - 315 WP_080865974.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FY149_RS12780 (FY149_12720) 505341..506357 + 1017 WP_163117905.1 ligase-associated DNA damage response exonuclease -
FY149_RS12785 (FY149_12725) 506359..507984 + 1626 WP_099085579.1 cisplatin damage response ATP-dependent DNA ligase -
FY149_RS12790 (FY149_12730) 508159..509124 + 966 WP_020813204.1 alpha/beta fold hydrolase -
FY149_RS12795 (FY149_12735) 509163..509945 - 783 WP_003508963.1 glycosyltransferase family 32 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-95)

Antitoxin

Uncharacterized protein HI1420

(6-97)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11772.48 Da        Isoelectric Point: 10.3857

>T149575 WP_080865974.1 NZ_CP048493:c505110-504796 [Agrobacterium tumefaciens]
MNSFQKSAEFDEWLKVLKDRAGKVRILHRLTAAEHGNFGDCAPVGEGVSEMRIHFGPGYRVYFTRVGKRVYFLLVGGDKS
SQRRDIKRAIEMAQTIGRDGRDGI

Download         Length: 315 bp

>T149575 NZ_CP064167:c2265103-2264789 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10765.29 Da        Isoelectric Point: 4.8307

>AT149575 WP_080865973.1 NZ_CP048493:c504815-504516 [Agrobacterium tumefaciens]
MAVTEFSKFDVSDYLDSDEAMIEFLAIAMEDENPDVFIGALGAVAKARGMSHIADASGLGRESLYKALRPGSKLRYETVQ
RVLHALGFRLAVEKSLNAA

Download         Length: 300 bp

>AT149575 NZ_CP064167:c2265339-2265106 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References