Detailed information of TA system TA149574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 460546..461090
• Replicon: chromosome
• Accession: NZ_CP048493
• Organism: Agrobacterium tumefaciens strain Tul001

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: FY149_RS12570
• Protein ID: WP_173992172.1
• Coordinates: 460800..461090 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A822V4A8
• Locus tag: FY149_RS12565
• Protein ID: WP_003509084.1
• Coordinates: 460546..460797 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY149_RS12545 (FY149_12485)	456307..456987	+	681	WP_003509096.1	GntR family transcriptional regulator	-
FY149_RS12550 (FY149_12490)	457033..457509	+	477	WP_099085593.1	DUF1284 domain-containing protein	-
FY149_RS12555 (FY149_12495)	457685..458785	-	1101	WP_099085592.1	hypothetical protein	-
FY149_RS12560 (FY149_12500)	459022..460167	+	1146	WP_087743499.1	site-specific DNA-methyltransferase	-
FY149_RS12565 (FY149_12505)	460546..460797	+	252	WP_003509084.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
FY149_RS12570 (FY149_12510)	460800..461090	+	291	WP_173992172.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY149_RS12575 (FY149_12515)	461132..461743	-	612	WP_087743505.1	HAD family phosphatase	-
FY149_RS12580 (FY149_12520)	461740..462852	-	1113	WP_099085591.1	A/G-specific adenine glycosylase	-
FY149_RS12585 (FY149_12525)	462980..463444	+	465	WP_020812858.1	hypothetical protein	-
FY149_RS12590 (FY149_12530)	463472..463993	+	522	WP_003509079.1	DUF721 domain-containing protein	-
FY149_RS12595 (FY149_12535)	464122..464802	+	681	WP_003509078.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (12-70)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11225.88 Da
• Isoelectric Point: 6.2182

>T149574 WP_173992172.1 NZ_CP048493:460800-461090 [Agrobacterium tumefaciens]
VGFRLSLLAEEDVIRIAEEGIAIFGLAQAQKYHRELYEIFELISLNPRMARERHELLPPLRIHRFRTHLVVYGVGADDDV
LIVRVRHAHEDWVNES

Nucleotide

• Length: 291 bp

>T149574 NZ_CP064167:2035947-2036207 [Escherichia coli O157:H7]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9339.55 Da
• Isoelectric Point: 9.7317

>AT149574 WP_003509084.1 NZ_CP048493:460546-460797 [Agrobacterium tumefaciens]
MATMNISLPAPMKEWVEAQSRTGRYSNASDYVRDLIRKDQERGEKIAAMQHFVDEGLQSGAGHRSQQALFAEAEARSMKR
KIG

Nucleotide

• Length: 252 bp

>AT149574 NZ_CP064167:2035668-2035928 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A822V4A8