Detailed information of TA system TA149572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 488600..489144
• Replicon: chromosome
• Accession: NZ_CP048481
• Organism: Agrobacterium tumefaciens strain Tul004

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: FY153_RS02455
• Protein ID: WP_173992172.1
• Coordinates: 488854..489144 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A822V4A8
• Locus tag: FY153_RS02450
• Protein ID: WP_003509084.1
• Coordinates: 488600..488851 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY153_RS02430 (FY153_02435)	484361..485041	+	681	WP_003509096.1	GntR family transcriptional regulator	-
FY153_RS02435 (FY153_02440)	485087..485563	+	477	WP_099085593.1	DUF1284 domain-containing protein	-
FY153_RS02440 (FY153_02445)	485739..486839	-	1101	WP_262513308.1	hypothetical protein	-
FY153_RS02445 (FY153_02450)	487076..488221	+	1146	WP_087743499.1	site-specific DNA-methyltransferase	-
FY153_RS02450 (FY153_02455)	488600..488851	+	252	WP_003509084.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
FY153_RS02455 (FY153_02460)	488854..489144	+	291	WP_173992172.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY153_RS02460 (FY153_02465)	489186..489797	-	612	WP_087743505.1	HAD family phosphatase	-
FY153_RS02465 (FY153_02470)	489794..490906	-	1113	WP_099085591.1	A/G-specific adenine glycosylase	-
FY153_RS02470 (FY153_02475)	491034..491498	+	465	WP_020812858.1	hypothetical protein	-
FY153_RS02475 (FY153_02480)	491526..492047	+	522	WP_003509079.1	DUF721 domain-containing protein	-
FY153_RS02480 (FY153_02485)	492176..492856	+	681	WP_003509078.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (12-70)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11225.88 Da
• Isoelectric Point: 6.2182

>T149572 WP_173992172.1 NZ_CP048481:488854-489144 [Agrobacterium tumefaciens]
VGFRLSLLAEEDVIRIAEEGIAIFGLAQAQKYHRELYEIFELISLNPRMARERHELLPPLRIHRFRTHLVVYGVGADDDV
LIVRVRHAHEDWVNES

Nucleotide

• Length: 291 bp

>T149572 NZ_CP064167:1773762-1773980 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9339.55 Da
• Isoelectric Point: 9.7317

>AT149572 WP_003509084.1 NZ_CP048481:488600-488851 [Agrobacterium tumefaciens]
MATMNISLPAPMKEWVEAQSRTGRYSNASDYVRDLIRKDQERGEKIAAMQHFVDEGLQSGAGHRSQQALFAEAEARSMKR
KIG

Nucleotide

• Length: 252 bp

>AT149572 NZ_CP064167:1773362-1773736 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A822V4A8