Detailed information of TA system TA149570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 2097375..2097981
• Replicon: chromosome
• Accession: NZ_CP048477
• Organism: Agrobacterium tumefaciens strain Yol001

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A822UZY8
• Locus tag: FY154_RS10510
• Protein ID: WP_020011654.1
• Coordinates: 2097375..2097671 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A822V132
• Locus tag: FY154_RS10515
• Protein ID: WP_060723677.1
• Coordinates: 2097676..2097981 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY154_RS10475 (FY154_10400)	2092970..2093317	+	348	WP_020809369.1	antibiotic biosynthesis monooxygenase	-
FY154_RS10480 (FY154_10405)	2093327..2093851	+	525	WP_020809370.1	heme utilization cystosolic carrier protein HutX	-
FY154_RS10485 (FY154_10410)	2094035..2094424	+	390	WP_240532256.1	hypothetical protein	-
FY154_RS10490 (FY154_10415)	2094577..2095116	+	540	WP_060723673.1	DUF2975 domain-containing protein	-
FY154_RS10495 (FY154_10420)	2095126..2095368	+	243	WP_060723674.1	helix-turn-helix transcriptional regulator	-
FY154_RS10500 (FY154_10425)	2095462..2096238	+	777	WP_020809374.1	YbaY family lipoprotein	-
FY154_RS10505 (FY154_10430)	2096445..2097329	+	885	WP_004429922.1	formyltetrahydrofolate deformylase	-
FY154_RS10510 (FY154_10435)	2097375..2097671	+	297	WP_020011654.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY154_RS10515 (FY154_10440)	2097676..2097981	+	306	WP_060723677.1	putative addiction module antidote protein	Antitoxin
FY154_RS10520 (FY154_10445)	2097995..2099389	-	1395	WP_065695049.1	sensor histidine kinase	-
FY154_RS10525 (FY154_10450)	2099386..2100066	-	681	WP_003491888.1	response regulator transcription factor	-
FY154_RS10530 (FY154_10455)	2100148..2101215	+	1068	WP_223566790.1	ABC transporter substrate-binding protein	-
FY154_RS10535 (FY154_10460)	2101641..2102585	+	945	WP_004429910.1	tripartite tricarboxylate transporter substrate binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10888.61 Da
• Isoelectric Point: 10.2733

>T149570 WP_020011654.1 NZ_CP048477:2097375-2097671 [Agrobacterium tumefaciens]
MLTIRETSEFADWLAGLRDVQARARIARRIYRLADGNPGDVKPVGEGVSELRIDLGPGYRVYFVRHGDVVIILLCGGDKA
SQPRDIARAKKLARALKE

Nucleotide

• Length: 297 bp

>T149570 NZ_CP064167:1152395-1152682 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10576.90 Da
• Isoelectric Point: 4.5580

>AT149570 WP_060723677.1 NZ_CP048477:2097676-2097981 [Agrobacterium tumefaciens]
MPLVTRDYDAARYLDSDEALAAYIADATESGDAQELAHALGVVARAKGMTDLSRQTGLPRQTLYKALSGEGNPELATIAK
VADALGYRLSLVAKPPADTAA

Nucleotide

• Length: 306 bp

>AT149570 NZ_CP064167:1152204-1152395 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA

Structures

Toxin

• AlphaFold DB: A0A822UZY8

Antitoxin

• AlphaFold DB: A0A822V132