Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 2097375..2097981 Replicon chromosome
Accession NZ_CP048473
Organism Agrobacterium tumefaciens strain Yol002

Toxin (Protein)


Gene name PumA Uniprot ID A0A822UZY8
Locus tag FY155_RS10505 Protein ID WP_020011654.1
Coordinates 2097375..2097671 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A822V132
Locus tag FY155_RS10510 Protein ID WP_060723677.1
Coordinates 2097676..2097981 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FY155_RS10470 (FY155_10405) 2092970..2093317 + 348 WP_020809369.1 antibiotic biosynthesis monooxygenase -
FY155_RS10475 (FY155_10410) 2093327..2093851 + 525 WP_020809370.1 heme utilization cystosolic carrier protein HutX -
FY155_RS10480 (FY155_10415) 2094035..2094424 + 390 WP_240532256.1 hypothetical protein -
FY155_RS10485 (FY155_10420) 2094577..2095116 + 540 WP_060723673.1 DUF2975 domain-containing protein -
FY155_RS10490 (FY155_10425) 2095126..2095368 + 243 WP_060723674.1 helix-turn-helix transcriptional regulator -
FY155_RS10495 (FY155_10430) 2095462..2096238 + 777 WP_020809374.1 YbaY family lipoprotein -
FY155_RS10500 (FY155_10435) 2096445..2097329 + 885 WP_004429922.1 formyltetrahydrofolate deformylase -
FY155_RS10505 (FY155_10440) 2097375..2097671 + 297 WP_020011654.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FY155_RS10510 (FY155_10445) 2097676..2097981 + 306 WP_060723677.1 putative addiction module antidote protein Antitoxin
FY155_RS10515 (FY155_10450) 2097995..2099389 - 1395 WP_065695049.1 sensor histidine kinase -
FY155_RS10520 (FY155_10455) 2099386..2100066 - 681 WP_003491888.1 response regulator transcription factor -
FY155_RS10525 (FY155_10460) 2100148..2101215 + 1068 WP_223566790.1 ABC transporter substrate-binding protein -
FY155_RS10530 (FY155_10465) 2101641..2102585 + 945 WP_004429910.1 tripartite tricarboxylate transporter substrate binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-97)

Antitoxin

Cro/C1-type helix-turn-helix domain

(47-90)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 10888.61 Da        Isoelectric Point: 10.2733

>T149565 WP_020011654.1 NZ_CP048473:2097375-2097671 [Agrobacterium tumefaciens]
MLTIRETSEFADWLAGLRDVQARARIARRIYRLADGNPGDVKPVGEGVSELRIDLGPGYRVYFVRHGDVVIILLCGGDKA
SQPRDIARAKKLARALKE

Download         Length: 297 bp

>T149565 NZ_CP064167:c156625-156518 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 10576.90 Da        Isoelectric Point: 4.5580

>AT149565 WP_060723677.1 NZ_CP048473:2097676-2097981 [Agrobacterium tumefaciens]
MPLVTRDYDAARYLDSDEALAAYIADATESGDAQELAHALGVVARAKGMTDLSRQTGLPRQTLYKALSGEGNPELATIAK
VADALGYRLSLVAKPPADTAA

Download         Length: 306 bp

>AT149565 NZ_CP064167:156673-156732 [Escherichia coli O157:H7]
GTCTGGTTTCAAGATTAGCCCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822UZY8

Antitoxin

Source ID Structure
AlphaFold DB A0A822V132

References