Detailed information of TA system TA149563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 458038..458582
• Replicon: chromosome
• Accession: NZ_CP048473
• Organism: Agrobacterium tumefaciens strain Yol002

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: FY155_RS02240
• Protein ID: WP_026363704.1
• Coordinates: 458292..458582 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A822V4A8
• Locus tag: FY155_RS02235
• Protein ID: WP_003509084.1
• Coordinates: 458038..458289 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY155_RS02210 (FY155_02210)	453796..454476	+	681	WP_003509096.1	GntR family transcriptional regulator	-
FY155_RS02215 (FY155_02215)	454522..454998	+	477	WP_099085593.1	DUF1284 domain-containing protein	-
FY155_RS02220 (FY155_02220)	455178..456278	-	1101	WP_020811573.1	hypothetical protein	-
FY155_RS02225 (FY155_02225)	456244..456456	+	213	WP_003509088.1	hypothetical protein	-
FY155_RS02230 (FY155_02230)	456515..457660	+	1146	WP_020010628.1	site-specific DNA-methyltransferase	-
FY155_RS02235 (FY155_02235)	458038..458289	+	252	WP_003509084.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
FY155_RS02240 (FY155_02240)	458292..458582	+	291	WP_026363704.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY155_RS02245 (FY155_02245)	458624..459235	-	612	WP_003509082.1	HAD family phosphatase	-
FY155_RS02250 (FY155_02250)	459232..460344	-	1113	WP_099085591.1	A/G-specific adenine glycosylase	-
FY155_RS02255 (FY155_02255)	460472..460936	+	465	WP_003509080.1	hypothetical protein	-
FY155_RS02260 (FY155_02260)	460964..461485	+	522	WP_003509079.1	DUF721 domain-containing protein	-
FY155_RS02265 (FY155_02265)	461614..462294	+	681	WP_003509078.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (12-70)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11195.86 Da
• Isoelectric Point: 6.2182

>T149563 WP_026363704.1 NZ_CP048473:458292-458582 [Agrobacterium tumefaciens]
VGFRLSLLAEEDVIRIAEEGIAIFGLAQAQKYHRELYEIFELISLNPRMARERHELLPPLRIHRFRAHLVVYGVGADDDV
LIVRVRHAHEDWVNES

Nucleotide

• Length: 291 bp

>T149563 NZ_CP064131:c67793-67377 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGATACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACATATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACAGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9339.55 Da
• Isoelectric Point: 9.7317

>AT149563 WP_003509084.1 NZ_CP048473:458038-458289 [Agrobacterium tumefaciens]
MATMNISLPAPMKEWVEAQSRTGRYSNASDYVRDLIRKDQERGEKIAAMQHFVDEGLQSGAGHRSQQALFAEAEARSMKR
KIG

Nucleotide

• Length: 252 bp

>AT149563 NZ_CP064131:c68020-67790 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGTGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGTTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A822V4A8