Detailed information of TA system TA149562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 336901..337387
• Replicon: chromosome
• Accession: NZ_CP048473
• Organism: Agrobacterium tumefaciens strain Yol002

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: FY155_RS01620
• Protein ID: WP_262514254.1
• Coordinates: 336901..337170 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A822V377
• Locus tag: FY155_RS01625
• Protein ID: WP_003503188.1
• Coordinates: 337154..337387 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY155_RS01585 (FY155_01590)	331923..332552	+	630	WP_020010519.1	DNA-3-methyladenine glycosylase I	-
FY155_RS01590 (FY155_01595)	332549..332995	+	447	WP_080865875.1	hypothetical protein	-
FY155_RS01595 (FY155_01600)	333011..334054	-	1044	WP_065694890.1	YeiH family protein	-
FY155_RS01600 (FY155_01605)	334136..335053	+	918	WP_026363679.1	LysR substrate-binding domain-containing protein	-
FY155_RS01605 (FY155_01610)	335264..335638	-	375	WP_262514251.1	DUF305 domain-containing protein	-
FY155_RS01610 (FY155_01615)	335696..336088	-	393	WP_262514252.1	hypothetical protein	-
FY155_RS01615 (FY155_01620)	336130..336894	-	765	WP_262514253.1	hypothetical protein	-
FY155_RS01620 (FY155_01625)	336901..337170	-	270	WP_262514254.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY155_RS01625 (FY155_01630)	337154..337387	-	234	WP_003503188.1	DUF6290 family protein	Antitoxin
FY155_RS01630 (FY155_01635)	337545..339068	+	1524	WP_262514255.1	histidine--tRNA ligase	-
FY155_RS01635 (FY155_01640)	339073..339441	+	369	WP_020010527.1	VOC family protein	-
FY155_RS01640 (FY155_01645)	339458..340585	+	1128	WP_262514256.1	ATP phosphoribosyltransferase regulatory subunit	-
FY155_RS01645 (FY155_01650)	340582..341274	+	693	WP_003503184.1	ATP phosphoribosyltransferase	-
FY155_RS01650 (FY155_01655)	341339..341785	-	447	WP_003503183.1	DoxX family protein	-
FY155_RS01655 (FY155_01660)	341942..342376	-	435	WP_003503182.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (6-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10283.91 Da
• Isoelectric Point: 10.8712

>T149562 WP_262514254.1 NZ_CP048473:c337170-336901 [Agrobacterium tumefaciens]
MIWKIEFQRAAVKQLGALTKSDANRIVSFLTGRIARDGNPRRTGAALQGSELGNFWRYRVGDYRIICDIQDHKLVVLVVE
IGHRREIYR

Nucleotide

• Length: 270 bp

>T149562 NZ_CP064129:c5027027-5026809 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 9212.31 Da
• Isoelectric Point: 4.6044

>AT149562 WP_003503188.1 NZ_CP048473:c337387-337154 [Agrobacterium tumefaciens]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRQRIANKERTYTLEEVMRDLDLEN

Nucleotide

• Length: 234 bp

>AT149562 NZ_CP064129:c5027428-5027054 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A822V377