Detailed information of TA system TA149556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 145311..145891
• Replicon: plasmid pAt
• Accession: NZ_CP048471
• Organism: Agrobacterium tumefaciens strain Yub001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FY156_RS28220
• Protein ID: WP_262528957.1
• Coordinates: 145508..145891 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FY156_RS28215
• Protein ID: WP_262528956.1
• Coordinates: 145311..145511 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY156_RS28180 (FY156_28010)	140777..141493	+	717	WP_262529016.1	L,D-transpeptidase	-
FY156_RS28185 (FY156_28015)	141845..142369	+	525	WP_262528947.1	thioredoxin family protein	-
FY156_RS28190 (FY156_28020)	142488..143135	+	648	WP_262528949.1	thioredoxin family protein	-
FY156_RS28195 (FY156_28025)	143137..143955	+	819	WP_262528951.1	TlpA disulfide reductase family protein	-
FY156_RS28200 (FY156_28030)	143952..144506	+	555	WP_262528953.1	hypothetical protein	-
FY156_RS28205 (FY156_28035)	144651..144797	-	147	Protein_158	type II toxin-antitoxin system RelE/ParE family toxin	-
FY156_RS28210 (FY156_28040)	144791..145099	-	309	WP_262528955.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
FY156_RS28215 (FY156_28045)	145311..145511	+	201	WP_262528956.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FY156_RS28220 (FY156_28050)	145508..145891	+	384	WP_262528957.1	type II toxin-antitoxin system VapC family toxin	Toxin
FY156_RS28225	146039..146320	-	282	WP_262528958.1	hypothetical protein	-
FY156_RS28230 (FY156_28055)	146405..146818	-	414	WP_262528857.1	hypothetical protein	-
FY156_RS28235 (FY156_28060)	147245..147517	+	273	WP_262528856.1	hypothetical protein	-
FY156_RS28240 (FY156_28065)	147674..148273	-	600	WP_262528959.1	hypothetical protein	-
FY156_RS28245 (FY156_28070)	148295..148612	-	318	WP_262528853.1	PepSY domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB / htpB	1..401476	401476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13969.02 Da
• Isoelectric Point: 6.4848

>T149556 WP_262528957.1 NZ_CP048471:145508-145891 [Agrobacterium tumefaciens]
VILVDSSIWIDHFRHGDSELTKIIGDDQLLCHPFVVGELALGSLRDRDAVLAFLAAQREAVVATHAEVMTVIDRYSVFSM
GIGYTDAHLLTSTLLDRRSSLWTRDKRLAAAAQKVGAVLYPSANIPH

Nucleotide

• Length: 384 bp

>T149556 NZ_CP064129:c3436088-3435720 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7241.37 Da
• Isoelectric Point: 10.2947

>AT149556 WP_262528956.1 NZ_CP048471:145311-145511 [Agrobacterium tumefaciens]
MRSTISLDDELMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPEAEVAPRRRSEVSK

Nucleotide

• Length: 201 bp

>AT149556 NZ_CP064129:c3436306-3436085 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA