Detailed information of TA system TA149555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 77376..77956
• Replicon: plasmid pAt
• Accession: NZ_CP048471
• Organism: Agrobacterium tumefaciens strain Yub001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FY156_RS27835
• Protein ID: WP_262528860.1
• Coordinates: 77376..77759 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FY156_RS27840
• Protein ID: WP_262528861.1
• Coordinates: 77756..77956 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY156_RS27805 (FY156_27645)	73694..74011	+	318	WP_262528853.1	PepSY domain-containing protein	-
FY156_RS27810 (FY156_27650)	74033..74632	+	600	WP_262528854.1	hypothetical protein	-
FY156_RS27815 (FY156_27655)	75126..75696	-	571	Protein_80	IS66 family transposase	-
FY156_RS27820 (FY156_27660)	75868..76140	-	273	WP_262528856.1	hypothetical protein	-
FY156_RS27825 (FY156_27665)	76567..76980	+	414	WP_262528857.1	hypothetical protein	-
FY156_RS27830	77065..77379	+	315	WP_262528859.1	hypothetical protein	-
FY156_RS27835 (FY156_27670)	77376..77759	-	384	WP_262528860.1	type II toxin-antitoxin system VapC family toxin	Toxin
FY156_RS27840 (FY156_27675)	77756..77956	-	201	WP_262528861.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FY156_RS27845 (FY156_27680)	78154..79016	-	863	Protein_86	IS30 family transposase	-
FY156_RS27850 (FY156_27685)	79139..80134	-	996	WP_262528862.1	LysR family transcriptional regulator	-
FY156_RS27855 (FY156_27690)	80228..81187	+	960	WP_262528863.1	MBL fold metallo-hydrolase	-
FY156_RS27860 (FY156_27695)	81312..82205	-	894	WP_262528864.1	LysR family transcriptional regulator	-
FY156_RS27865 (FY156_27700)	82348..82914	+	567	WP_262528866.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB / htpB	1..401476	401476
	-	flank	IS/Tn	-	-	78252..79016	764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13910.98 Da
• Isoelectric Point: 6.7537

>T149555 WP_262528860.1 NZ_CP048471:c77759-77376 [Agrobacterium tumefaciens]
VILVDSSIWIDHFRHGGSELTKIIGDDQLLCHPFVVGELALGSLRDRDAVLAFLAAQREAVVATHAEVMTVIDRYSVFSM
GIGYTDAHLLTSTLLDRRSSLWTRDKRLAAAAQKVGAVLYPSANIPH

Nucleotide

• Length: 384 bp

>T149555 NZ_CP064129:c3410117-3409770 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
ATTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7243.45 Da
• Isoelectric Point: 10.7407

>AT149555 WP_262528861.1 NZ_CP048471:c77956-77756 [Agrobacterium tumefaciens]
MRSTISLDDKLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPEAEVAPRRRSEVSM

Nucleotide

• Length: 201 bp

>AT149555 NZ_CP064129:c3409770-3409471 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGATAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA