Detailed information of TA system TA149554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 60208..60878
• Replicon: plasmid pAt
• Accession: NZ_CP048471
• Organism: Agrobacterium tumefaciens strain Yub001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FY156_RS27735
• Protein ID: WP_262528840.1
• Coordinates: 60459..60878 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FY156_RS27730
• Protein ID: WP_262528617.1
• Coordinates: 60208..60462 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY156_RS27690 (FY156_27530)	55333..56049	+	717	WP_262528834.1	hypothetical protein	-
FY156_RS27695 (FY156_27535)	55988..56311	+	324	WP_262528835.1	hypothetical protein	-
FY156_RS27700 (FY156_27540)	56415..56699	-	285	Protein_57	hypothetical protein	-
FY156_RS27705 (FY156_27545)	56701..56796	-	96	Protein_58	MarC family protein	-
FY156_RS27710 (FY156_27550)	56946..57197	+	252	WP_262528836.1	antitoxin	-
FY156_RS27715 (FY156_27555)	57194..57505	+	312	WP_262528837.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FY156_RS27720 (FY156_27560)	58013..59107	+	1095	WP_262528838.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
FY156_RS27725 (FY156_27565)	59109..59996	+	888	WP_262528839.1	antitoxin	-
FY156_RS27730 (FY156_27570)	60208..60462	+	255	WP_262528617.1	plasmid stabilization protein	Antitoxin
FY156_RS27735 (FY156_27575)	60459..60878	+	420	WP_262528840.1	type II toxin-antitoxin system VapC family toxin	Toxin
FY156_RS27740 (FY156_27580)	61145..61534	-	390	WP_262528841.1	hypothetical protein	-
FY156_RS27745 (FY156_27585)	61629..63209	-	1581	WP_262528842.1	chaperonin GroEL	-
FY156_RS27750 (FY156_27590)	63276..63572	-	297	WP_262529009.1	co-chaperone GroES	-
FY156_RS27755 (FY156_27595)	64122..64931	-	810	WP_262528843.1	helix-turn-helix transcriptional regulator	-
FY156_RS27760 (FY156_27600)	65015..65443	+	429	WP_262528844.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB / htpB	1..401476	401476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14974.23 Da
• Isoelectric Point: 5.1085

>T149554 WP_262528840.1 NZ_CP048471:60459-60878 [Agrobacterium tumefaciens]
MILLDTNVISEPWKSAPDPAVLTWLDAQAIETLFISAISIAELRFGIASMPVGKRQTILHDRLESEVLPLFDGRILPFSV
GTSHFYAALMADGRKSGKAIAQADGYIAATAAENHLAVATRDTSPFEAAGLRVINPWLA

Nucleotide

• Length: 420 bp

>T149554 NZ_CP064129:c2980423-2980013 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9212.31 Da
• Isoelectric Point: 6.8809

>AT149554 WP_262528617.1 NZ_CP048471:60208-60462 [Agrobacterium tumefaciens]
MAAVTIRNISDETHRALRARAAHHGRSTEAEIRDILEAAVRPSGRVKLGSLLAAIGRDAELSNSDVEALHQQRDRTPAEP
MTFE

Nucleotide

• Length: 255 bp

>AT149554 NZ_CP064129:c2980670-2980404 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA