Detailed information of TA system TA149545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 931336..933083
• Replicon: chromosome
• Accession: NZ_CP048461
• Organism: Burkholderia multivorans strain P1Bm2009

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: G0D86_RS19625
• Protein ID: WP_048804598.1
• Coordinates: 931336..932232 (+)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B9AZJ2
• Locus tag: G0D86_RS19630
• Protein ID: WP_006396780.1
• Coordinates: 932229..933083 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0D86_RS19595	926736..927815	-	1080	WP_039217461.1	porin	-
G0D86_RS19600	928251..928568	+	318	WP_155825139.1	non-heme iron oxygenase ferredoxin subunit	-
G0D86_RS19605	928570..928881	+	312	WP_006396775.1	hypothetical protein	-
G0D86_RS19610	928929..929999	+	1071	WP_006396776.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
G0D86_RS19615	929992..930762	+	771	WP_006413827.1	SDR family oxidoreductase	-
G0D86_RS19620	930790..931317	+	528	WP_006396778.1	cupin domain-containing protein	-
G0D86_RS19625	931336..932232	+	897	WP_048804598.1	alpha/beta fold hydrolase	Toxin
G0D86_RS19630	932229..933083	+	855	WP_006396780.1	IclR family transcriptional regulator	Antitoxin
G0D86_RS19635	933080..933877	+	798	WP_155825136.1	SDR family oxidoreductase	-
G0D86_RS19640	933870..935537	+	1668	WP_206381835.1	thiamine pyrophosphate-binding protein	-
G0D86_RS19645	935530..936360	+	831	WP_006396783.1	aspartate dehydrogenase	-
G0D86_RS19650	936422..937912	+	1491	WP_043278809.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (51-280)

Antitoxin

• Transcription regulator IclR, N-terminal (23-71)

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 30583.83 Da
• Isoelectric Point: 8.0534

>T149545 WP_048804598.1 NZ_CP048461:931336-932232 [Burkholderia multivorans]
MSVVDKRERDRTAAFATLLERFPEQRCAAGAAGTIGYREAGAQHAGAALPVVLLHGIGSGAASWVRQLDALGASRRVLAW
DAPGYGASTPVHGASPSAVDYAASLDAWLDALGIARCVLVGHSLGAIVAGGMVRVKPERIAGLLLISPAGGYGSAPAETR
ASRRDARLAMLAELGPAGLAAQRSGNMLSGFATEDARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVG
ANDAITPPAACERIAAAAGVGLQVIPQAGHAGYVEAPDVYSALIDTFCRQCDIERRAA

Nucleotide

• Length: 897 bp

>T149545 NZ_CP064127:c2945029-2944604 [Yersinia pestis]
GTGGCCCAGTGGCGATGTAACCTGCGGGTATCCTGGCATACTCAACTCTTTTCTTTATTGGCACACGGCATATTGGTCAT
TTTAACCTTGGTCGCGCCCTGGCCCTTGGGTTATACCGCCCTATGGCTGGTATTGCTGACGTTAGTGGTGTTTGAGTGTA
TCCGCAGCCAGAAAAGGATTAAATCCTGTCAGGGGGAAATCAGGCTAAAACCTGGCAACCTGGTGCGATGGAAAAGGCAT
GAATGGACCGTGGTCAAGCCGCCGTGGATCACCCGCTATGGGGTATTATTACATTTACAACAAACAGGCGGCCACGCGAC
CCGAAAGCGTCTATGGCTGTCCGCTGACAGCATGTCTGAAGATGAGTGGCGGCAGCTATGCCTGTTGCTGCGGCATTCAT
TTGAATCTGACGATGGAACGATGTGA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31176.75 Da
• Isoelectric Point: 6.6558

>AT149545 WP_006396780.1 NZ_CP048461:932229-933083 [Burkholderia multivorans]
MSEPLQSAAEPAKAEASYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYKL
GIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQSEAPVFSSIRVNVGTRLPAHATTHGHV
LMGDLSLDELRALYPEGTLPRMTNATPDTVDALYTMIREDALRGYGISNSSFERGISVVTAPVRNDTQKIVACITVTVPR
PEIDATLIADGLIDKVRRAAAELSRRLNYRSDDEHTFLKALGLR

Nucleotide

• Length: 855 bp

>AT149545 NZ_CP064127:c2945276-2945010 [Yersinia pestis]
ATGGAAATTGATAATAAAGCCCGTATTCACTGGGCATGCCGCCGTGGTATGCGTGAACTTGATATCTCCATCATGCCGTT
TTTTGAATATGAATATGATAGCTTGAGCGATAACGAGAAACAGGCATTTATCCGCTTGCTTGAGTGTGATGACCCAGATT
TATTCAACTGGCTGATGAACCACGGGGAGCCGCAGGATAGCGAGCTATATCAGATGGTCAAACTGATTCAATCACGAAAT
AAAGCCCGTGGCCCAGTGGCGATGTAA

Structures

Antitoxin

• AlphaFold DB: B9AZJ2