Detailed information of TA system TA149537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 931291..933038
• Replicon: chromosome
• Accession: NZ_CP048458
• Organism: Burkholderia multivorans strain P1Bm2011a

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: G0D87_RS19645
• Protein ID: WP_048804598.1
• Coordinates: 931291..932187 (+)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B9AZJ2
• Locus tag: G0D87_RS19650
• Protein ID: WP_006396780.1
• Coordinates: 932184..933038 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0D87_RS19615	926691..927770	-	1080	WP_039217461.1	porin	-
G0D87_RS19620	928206..928523	+	318	WP_155825139.1	non-heme iron oxygenase ferredoxin subunit	-
G0D87_RS19625	928525..928836	+	312	WP_006396775.1	hypothetical protein	-
G0D87_RS19630	928884..929954	+	1071	WP_006396776.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
G0D87_RS19635	929947..930717	+	771	WP_006413827.1	SDR family oxidoreductase	-
G0D87_RS19640	930745..931272	+	528	WP_006396778.1	cupin domain-containing protein	-
G0D87_RS19645	931291..932187	+	897	WP_048804598.1	alpha/beta fold hydrolase	Toxin
G0D87_RS19650	932184..933038	+	855	WP_006396780.1	IclR family transcriptional regulator	Antitoxin
G0D87_RS19655	933035..933832	+	798	WP_155825136.1	SDR family oxidoreductase	-
G0D87_RS19660	933825..935492	+	1668	WP_206381835.1	thiamine pyrophosphate-binding protein	-
G0D87_RS19665	935485..936315	+	831	WP_006396783.1	aspartate dehydrogenase	-
G0D87_RS19670	936377..937867	+	1491	WP_043278809.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (51-280)

Antitoxin

• Transcription regulator IclR, N-terminal (23-71)

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 30583.83 Da
• Isoelectric Point: 8.0534

>T149537 WP_048804598.1 NZ_CP048458:931291-932187 [Burkholderia multivorans]
MSVVDKRERDRTAAFATLLERFPEQRCAAGAAGTIGYREAGAQHAGAALPVVLLHGIGSGAASWVRQLDALGASRRVLAW
DAPGYGASTPVHGASPSAVDYAASLDAWLDALGIARCVLVGHSLGAIVAGGMVRVKPERIAGLLLISPAGGYGSAPAETR
ASRRDARLAMLAELGPAGLAAQRSGNMLSGFATEDARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVG
ANDAITPPAACERIAAAAGVGLQVIPQAGHAGYVEAPDVYSALIDTFCRQCDIERRAA

Nucleotide

• Length: 897 bp

>T149537 NZ_CP064126:2452884-2453066 [Yersinia pestis]
GTGAAAAGTTCGGAGCTGATAAAACTGCTCGAAACAAATGGTTGGAAGTTGATGAGAGTTAAAGGCAGTCATCATCAATT
CAAACATCCTGATTTCGCGGCAGTCATAACAGTACCGCATCCCAAAAAGGATGTTAAACCGGGAACCCTCCGGCAAATTC
TGAAAGATGCAAACCTTAGTTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31176.75 Da
• Isoelectric Point: 6.6558

>AT149537 WP_006396780.1 NZ_CP048458:932184-933038 [Burkholderia multivorans]
MSEPLQSAAEPAKAEASYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYKL
GIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQSEAPVFSSIRVNVGTRLPAHATTHGHV
LMGDLSLDELRALYPEGTLPRMTNATPDTVDALYTMIREDALRGYGISNSSFERGISVVTAPVRNDTQKIVACITVTVPR
PEIDATLIADGLIDKVRRAAAELSRRLNYRSDDEHTFLKALGLR

Nucleotide

• Length: 855 bp

>AT149537 NZ_CP064126:2453111-2453530 [Yersinia pestis]
ATGGCTATCTATCCCGCCTATGTTCACGTTGATAATGATGGCTCGGCAAGCGGTTATTTCCCCGATGTCAAAGGCTGTAT
TTTTGCCATCAATGCCGGGGAGGATTTATTTGCAGAAGCATCAAGCGCCCTGGATGCACACTTTGAAGCTTTGGTGTCTG
AAGGTATCGAAATTCCTGAAGCTCATGACATGCCTTACCACGTTTACCGTAACCCATGCGATTACGCTGATGGCGGACAG
TGGTATAACGTGAATATTGATATGTCGAAATATGATGGAAAAGTTGAACGAATTAACGTCACTCTTCCGCACCGGCTGAT
TCATCAAATCGATACTATTGTTAAAGTGCGTCCCGAGTATGCCAGCCGAAGCAATTTTCTAGCCGAAGCAGCACGTAAAG
AATTACAGAAATTGGCGTAA

Structures

Antitoxin

• AlphaFold DB: B9AZJ2