Detailed information of TA system TA149521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 930150..931897
• Replicon: chromosome
• Accession: NZ_CP048452
• Organism: Burkholderia multivorans strain P1Bm2012b

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: G0D89_RS19630
• Protein ID: WP_048804598.1
• Coordinates: 930150..931046 (+)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B9AZJ2
• Locus tag: G0D89_RS19635
• Protein ID: WP_006396780.1
• Coordinates: 931043..931897 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0D89_RS19600	925550..926629	-	1080	WP_039217461.1	porin	-
G0D89_RS19605	927065..927382	+	318	WP_155825139.1	non-heme iron oxygenase ferredoxin subunit	-
G0D89_RS19610	927384..927695	+	312	WP_006396775.1	hypothetical protein	-
G0D89_RS19615	927743..928813	+	1071	WP_006396776.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
G0D89_RS19620	928806..929576	+	771	WP_006413827.1	SDR family oxidoreductase	-
G0D89_RS19625	929604..930131	+	528	WP_006396778.1	cupin domain-containing protein	-
G0D89_RS19630	930150..931046	+	897	WP_048804598.1	alpha/beta fold hydrolase	Toxin
G0D89_RS19635	931043..931897	+	855	WP_006396780.1	IclR family transcriptional regulator	Antitoxin
G0D89_RS19640	931894..932691	+	798	WP_155825136.1	SDR family oxidoreductase	-
G0D89_RS19645	932684..934351	+	1668	WP_206381835.1	thiamine pyrophosphate-binding protein	-
G0D89_RS19650	934344..935174	+	831	WP_006396783.1	aspartate dehydrogenase	-
G0D89_RS19655	935236..936726	+	1491	WP_043278809.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (51-280)

Antitoxin

• Transcription regulator IclR, N-terminal (23-71)

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 30583.83 Da
• Isoelectric Point: 8.0534

>T149521 WP_048804598.1 NZ_CP048452:930150-931046 [Burkholderia multivorans]
MSVVDKRERDRTAAFATLLERFPEQRCAAGAAGTIGYREAGAQHAGAALPVVLLHGIGSGAASWVRQLDALGASRRVLAW
DAPGYGASTPVHGASPSAVDYAASLDAWLDALGIARCVLVGHSLGAIVAGGMVRVKPERIAGLLLISPAGGYGSAPAETR
ASRRDARLAMLAELGPAGLAAQRSGNMLSGFATEDARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVG
ANDAITPPAACERIAAAAGVGLQVIPQAGHAGYVEAPDVYSALIDTFCRQCDIERRAA

Nucleotide

• Length: 897 bp

>T149521 NZ_CP064123:3330027-3330323 [Yersinia pestis]
ATGGAGAAACGGACTCCGCATACTCGTTTATTAAAAGTAAAAGAATTGGTATTGAAGGGGAATATTAAAACGACACGAAC
TGCCAGAGATGGGGCTGAGGAACTGGGACTGAGTTTTAGAGATATGTGTGATGCAGTTTCCGAACTGATTAGCGCCGATT
TTTATAAAAGTATGACGACACATCAAGATCATACCATTTGGCAGGATGTTTACCGTCCTATGCTTTCTTGCGGTAGAGTT
TATTTGAAAATCACGGTTATCGATGATGTTTTGAATGTCTCGTTTAAGGAGATTTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31176.75 Da
• Isoelectric Point: 6.6558

>AT149521 WP_006396780.1 NZ_CP048452:931043-931897 [Burkholderia multivorans]
MSEPLQSAAEPAKAEASYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYKL
GIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQSEAPVFSSIRVNVGTRLPAHATTHGHV
LMGDLSLDELRALYPEGTLPRMTNATPDTVDALYTMIREDALRGYGISNSSFERGISVVTAPVRNDTQKIVACITVTVPR
PEIDATLIADGLIDKVRRAAAELSRRLNYRSDDEHTFLKALGLR

Nucleotide

• Length: 855 bp

>AT149521 NZ_CP064123:3330323-3330721 [Yersinia pestis]
ATGATGAAATGTCCAGTTTGTGGCGGTGTAGAATTAATCCACGAAGCGCGTGACGTTCCCTTTACCTATAAAGGGCGTAA
GTTCATCGTCGAAGGCGTGATAGGGCAACATTGCCCGGCATGCGGTGAAACAGTCATGACCGAAGCCGAGTCCGATGTCT
ATGCAGCAAAAACAATGCTTTTGCGAAAAATGGTCAATACAGAATCGATTGCCCCCGCATATATTGTGCAGATCCGAAAA
AAGTTGGGCCTAAACCAGCGGGAAGCCAGTGAGATTTTTGGTGGTGGGGTTAATGCATTTTCGCGTTACGAAAAAGGTAA
GGCTTTGCCACATCCATCAACGATAAAACTGTTGCAAGTGCTTGATAAACACCCGGAGTTATTGAATGAAATTAGGTAG

Structures

Antitoxin

• AlphaFold DB: B9AZJ2