Detailed information of TA system TA149500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 120829..121475
• Replicon: plasmid unnamed
• Accession: NZ_CP048444
• Organism: Burkholderia multivorans strain P2Bm2011a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G0D92_RS30000
• Protein ID: WP_012211060.1
• Coordinates: 121068..121475 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G0D92_RS29995
• Protein ID: WP_009692911.1
• Coordinates: 120829..121071 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0D92_RS29975	115966..116823	-	858	WP_012211056.1	replication initiator protein A	-
G0D92_RS29980	117566..118531	-	966	WP_012211057.1	ParB/RepB/Spo0J family partition protein	-
G0D92_RS29985	118531..119202	-	672	WP_206382236.1	AAA family ATPase	-
G0D92_RS29990	119697..120727	+	1031	Protein_107	non-homologous end-joining DNA ligase	-
G0D92_RS29995	120829..121071	+	243	WP_009692911.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
G0D92_RS30000	121068..121475	+	408	WP_012211060.1	type II toxin-antitoxin system VapC family toxin	Toxin
G0D92_RS30005	121819..122139	+	321	WP_105839319.1	hypothetical protein	-
G0D92_RS30010	122243..122779	+	537	WP_105839318.1	hypothetical protein	-
G0D92_RS30015	122821..123057	+	237	Protein_112	hypothetical protein	-
G0D92_RS30020	123054..123785	+	732	WP_105839315.1	hypothetical protein	-
G0D92_RS30025	123787..126123	+	2337	WP_105839314.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..161953	161953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (12-49)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14613.77 Da
• Isoelectric Point: 5.1402

>T149500 WP_012211060.1 NZ_CP048444:121068-121475 [Burkholderia multivorans]
MSLYLLDTNILSNVIRDPRGACASRIGETRPEQVCTSIVVAAELRYGVCKRGSSALAQRVEQLLASLTVLPLQSDADQCY
GRLRADLERQGQLIGANDMLIAAHAMAVDAVLVTDNTAEFTRVTGLAVENWLRPA

Nucleotide

• Length: 408 bp

>T149500 NZ_CP064120:2667178-2667636 [Yersinia pestis]
ATGACGCTATACCGCATTGTTATGCGCCGCTATCTTGCATCGGCATGGACTGGTTACGGCGCTGAAATCTATGGTGGTCG
TTGGAATCATAAAGGCCATGCTGCCATCTATTTAGCCAGCAGTATTTCATTGGCTATGCTCGAAACACTGGCACATATTC
AGGACAGTTCAACACTCAGCGAATTTGAATTATTCCAAATTGAAATCAGTGACAGCAGTATTATGTTGCTACAGCCGCAG
GACTGGCCCACGGATTGGCGTAGCGATCCGGCACCGGTGGCAACGATGGATGTCGGCACCGAGTGGCTGGAATCAGAATC
CTCGGTAGGCTTACTGGTCCCGTCCACGTTGGTGCCGTCAGAAAACAACCTACTGGTCAATCCTCATCACAAAGATTTTC
CAGCGTGTTTGGCCAGCGTCAAACCGCTCTCTTTTGTCTTTGATCCACGGTTAAAATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9309.54 Da
• Isoelectric Point: 4.8615

>AT149500 WP_009692911.1 NZ_CP048444:120829-121071 [Burkholderia multivorans]
MPTHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAPSLRELFAQWAPLDETFPDIENLPAEPVDIE

Nucleotide

• Length: 243 bp

>AT149500 NZ_CP064120:2666670-2667116 [Yersinia pestis]
ATGAGAGCGTACCGCCCAAACCCCGCGACTAAAATGGGAACGCTTTGGCGTGAAATTGGCTTACCCGCCAGCCGCGGCAC
GATATTGGTCGATAGTATCAAAATGGGGTTTTCTGTTGATGTTATCGACAGCATTCATTTATGGGCATCCATGCCTAAAG
CTGAAATATTACGTGCTACCGGTATCCCCAGCCGTAGCCTGACTCGCCGCCGCACACATGATGGCCGTTTTACGCCTGAA
GAAAGCGAGAGAATCGCACGTTTTGTCCGCGTAATGGATGCCGCTGTCGATTTGTTCGGCGGCGATAAAGGTAAAGCCAT
TACCTGGATGTCCACGCCCATCAAAGGATTGGGGTACCGCAGCCCAGACTCCTTACTGGAAACCGAAACTGGCGCACTGG
AAGTGTGTGATTTGATTGGTCGTTTGGAACACGGCGTCTTTACTTAA