Detailed information of TA system TA149481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4006099..4006913
• Replicon: chromosome
• Accession: NZ_CP048439
• Organism: Escherichia coli strain NBRC 3301

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: GXM24_RS19500
• Protein ID: WP_001054376.1
• Coordinates: 4006099..4006356 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: GXM24_RS19505
• Protein ID: WP_001309181.1
• Coordinates: 4006368..4006913 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GXM24_RS19480	4001387..4002493	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
GXM24_RS19485	4002558..4003538	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
GXM24_RS19490	4004121..4005361	-	1241	Protein_3799	helicase YjhR	-
GXM24_RS19495	4005477..4005722	+	246	Protein_3800	GNAT family N-acetyltransferase	-
GXM24_RS19500	4006099..4006356	+	258	WP_001054376.1	hypothetical protein	Toxin
GXM24_RS19505	4006368..4006913	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
GXM24_RS19510	4006969..4007715	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
GXM24_RS19515	4007884..4008102	+	219	Protein_3804	hypothetical protein	-
GXM24_RS23135	4008140..4008256	+	117	Protein_3805	VOC family protein	-
GXM24_RS19520	4008501..4009622	+	1122	WP_000010829.1	M42 family metallopeptidase	-
GXM24_RS19525	4009619..4009897	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
GXM24_RS19530	4009909..4011222	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimE / fimB	3997519..4051591	54072

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T149481 WP_001054376.1 NZ_CP048439:4006099-4006356 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T149481 NZ_CP064117:919874-920170 [Yersinia pestis]
ATGGAGAAACGGACTCCGCATACTCGTTTATTAAAAGTAAAAGAATTGGTATTGAAGGGGAATATTAAAACGACACGAAC
TGCCAGAGATGGGGCTGAGGAACTGGGACTGAGTTTTAGAGATATGTGTGATGCAGTTTCCGAACTGATTAGCGCCGATT
TTTATAAAAGTATGACGACACATCAAGATCATACCATTTGGCAGGATGTTTACCGTCCTATGCTTTCTTGCGGTAGAGTT
TATTTGAAAATCACGGTTATCGATGATGTTTTGAATGTCTCGTTTAAGGAGATTTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT149481 WP_001309181.1 NZ_CP048439:4006368-4006913 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT149481 NZ_CP064117:920170-920568 [Yersinia pestis]
ATGATGAAATGTCCAGTTTGTGGCGGTGTAGAATTAATCCACGAAGCGCGTGACGTTCCCTTTACCTATAAAGGGCGTAA
GTTCATCGTCGAAGGCGTGATAGGGCAACATTGCCCGGCATGCGGTGAAACAGTCATGACCGAAGCCGAGTCCGATGTCT
ATGCAGCAAAAACAATGCTTTTGCGAAAAATGGTCAATACAGAATCGATTGCCCCCGCATATATTGTGCAGATCCGAAAA
AAGTTGGGCCTAAACCAGCGGGAAGCCAGTGAGATTTTTGGTGGTGGGGTTAATGCATTTTCGCGTTACGAAAAAGGTAA
GGCTTTGCCACATCCATCAACGATAAAACTGTTGCAAGTGCTTGATAAACACCCGGAGTTATTGAATGAAATTAGGTAG

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8