Detailed information of TA system TA149476

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3635269..3635948
• Replicon: chromosome
• Accession: NZ_CP048439
• Organism: Escherichia coli strain NBRC 3301

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: GXM24_RS17775
• Protein ID: WP_000854672.1
• Coordinates: 3635607..3635948 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: GXM24_RS17770
• Protein ID: WP_000070395.1
• Coordinates: 3635269..3635586 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GXM24_RS17720	3630316..3631179	+	864	WP_001065553.1	GTPase family protein	-
GXM24_RS17725	3631271..3632092	+	822	WP_000197389.1	DUF945 domain-containing protein	-
GXM24_RS17730	3632309..3632500	+	192	Protein_3455	DeoR family transcriptional regulator	-
GXM24_RS17735	3632496..3632723	+	228	WP_001548158.1	hypothetical protein	-
GXM24_RS17740	3632723..3633166	+	444	WP_000824223.1	hypothetical protein	-
GXM24_RS17745	3633189..3633656	+	468	WP_001547765.1	hypothetical protein	-
GXM24_RS17750	3633733..3633972	+	240	WP_000194654.1	DUF905 domain-containing protein	-
GXM24_RS17755	3634070..3634528	+	459	WP_000211838.1	antirestriction protein	-
GXM24_RS17760	3634544..3635020	+	477	WP_000811693.1	RadC family protein	-
GXM24_RS17765	3635029..3635250	+	222	WP_000691994.1	DUF987 domain-containing protein	-
GXM24_RS17770	3635269..3635586	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
GXM24_RS17775	3635607..3635948	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
GXM24_RS17785	3636520..3637773	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
GXM24_RS17790	3637785..3638888	-	1104	WP_001285288.1	glutamate 5-kinase	-
GXM24_RS17795	3639176..3640231	+	1056	WP_000749863.1	phosphoporin PhoE	-
GXM24_RS17800	3640270..3640671	-	402	WP_000174689.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3602255..3676886	74631

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T149476 WP_000854672.1 NZ_CP048439:3635607-3635948 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T149476 NZ_CP064113:c3666280-3665870 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTGGTGTTTGATAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAGGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGACAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGGGATCTGCGCCGGCTAATCGTGCAACAGCCGA
CGCAGGAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT149476 WP_000070395.1 NZ_CP048439:3635269-3635586 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT149476 NZ_CP064113:c3666527-3666261 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684