Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2340778..2341416 Replicon chromosome
Accession NZ_CP048439
Organism Escherichia coli strain NBRC 3301

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GXM24_RS11305 Protein ID WP_000813794.1
Coordinates 2341240..2341416 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GXM24_RS11300 Protein ID WP_001270286.1
Coordinates 2340778..2341194 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GXM24_RS11280 2335930..2336871 - 942 WP_001251304.1 ABC transporter permease -
GXM24_RS11285 2336872..2337885 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
GXM24_RS11290 2337903..2339048 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
GXM24_RS11295 2339293..2340699 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
GXM24_RS11300 2340778..2341194 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GXM24_RS11305 2341240..2341416 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GXM24_RS11310 2341638..2341868 + 231 WP_000494244.1 YncJ family protein -
GXM24_RS11315 2341960..2343921 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GXM24_RS11320 2343994..2344530 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
GXM24_RS11325 2344583..2345797 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T149474 WP_000813794.1 NZ_CP048439:c2341416-2341240 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T149474 NZ_CP064113:c2221125-2220640 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACCTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGGAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCAGTTGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATTATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT149474 WP_001270286.1 NZ_CP048439:c2341194-2340778 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT149474 NZ_CP064113:c2221379-2221113 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCGGCCATAGAAAAACTGCTG
GCAAGGAAACCACAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References