Detailed information of TA system TA149461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 1095881..1097318
• Replicon: chromosome
• Accession: NZ_CP048439
• Organism: Escherichia coli strain NBRC 3301

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: GXM24_RS05275
• Protein ID: WP_000155570.1
• Coordinates: 1096245..1097318 (-)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: GXM24_RS05270
• Protein ID: WP_000461704.1
• Coordinates: 1095881..1096252 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GXM24_RS05235	1091082..1091396	+	315	WP_000065803.1	arsenate reductase	-
GXM24_RS05240	1091621..1092088	-	468	WP_000702495.1	hypothetical protein	-
GXM24_RS05245	1092112..1092555	-	444	WP_000824222.1	hypothetical protein	-
GXM24_RS05250	1092555..1092791	-	237	WP_001144029.1	hypothetical protein	-
GXM24_RS05255	1092832..1093533	-	702	WP_000189409.1	WYL domain-containing protein	-
GXM24_RS05260	1093750..1094571	-	822	WP_000197391.1	DUF945 domain-containing protein	-
GXM24_RS05265	1094663..1095526	-	864	WP_000787597.1	GTPase family protein	-
GXM24_RS05270	1095881..1096252	-	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
GXM24_RS05275	1096245..1097318	-	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
GXM24_RS05280	1097460..1097723	+	264	WP_000502050.1	DUF1524 domain-containing protein	-
GXM24_RS05285	1098083..1099699	+	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
GXM24_RS05290	1099696..1101885	+	2190	WP_000136402.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T149461 WP_000155570.1 NZ_CP048439:c1097318-1096245 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T149461 NZ_CP064108:1259731-1260141 [Klebsiella oxytoca]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAATGGTTCTCACTGTTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGGTGTTGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGACAGGGGGAAATAAAGCTGCTGATTGACTCTCGCCTGCGCTGGCAAAAAGCG
GAGTGGGATATCGTCGGCACCCCGTGGGTAATCAATAGCGGAATGCTGCTGCGTCTGCGAAATACGGAAAACCAACGCAC
TCAGCATCTATGGGTGGCGGCGGATAGCATGGACGCGGGCGAGTGGCGCGATCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGATTAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT149461 WP_000461704.1 NZ_CP048439:c1096252-1095881 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT149461 NZ_CP064108:1259484-1259750 [Klebsiella oxytoca]
ATGGATATTAACAATAAAGCTCGCATCCACTGGGCATGCCGCCGGGGAATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTTGAGCATGAGTACGATACGCTCAGTGATGACGATAAGCGGTTGTTCATCCGCCTGCTGGAAAATGACGATCCGGACC
TGTTCAACTGGTTGATGAACCATGGCGAACCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• PDB: 6Y2P
• AlphaFold DB: A0A373F4G4