Detailed information of TA system TA149449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FaRel2-ATfaRel2/-
• Location: 142591..143419
• Replicon: chromosome
• Accession: NZ_CP048436
• Organism: Flavonifractor plautii strain JCM 32125

Toxin (Protein)

• Gene name: faRel2
• Uniprot ID: -
• Locus tag: GXM20_RS00740
• Protein ID: WP_039997549.1
• Coordinates: 142591..143208 (+)
• Length: 206 a.a.

Antitoxin (Protein)

• Gene name: ATfaRel2
• Uniprot ID: G9YUB0
• Locus tag: GXM20_RS00745
• Protein ID: WP_007493440.1
• Coordinates: 143189..143419 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GXM20_RS00720	138679..139722	+	1044	WP_007493453.1	ketopantoate reductase family protein	-
GXM20_RS00725	139784..141103	+	1320	WP_007493451.1	GntP family permease	-
GXM20_RS00730	141272..141973	+	702	WP_007493446.1	N-acyl homoserine lactonase family protein	-
GXM20_RS00735	141992..142459	+	468	WP_007493444.1	hypothetical protein	-
GXM20_RS00740	142591..143208	+	618	WP_039997549.1	hypothetical protein	Toxin
GXM20_RS00745	143189..143419	+	231	WP_007493440.1	hypothetical protein	Antitoxin
GXM20_RS19790	143970..144293	-	324	WP_007493435.1	hypothetical protein	-
GXM20_RS00755	144535..145107	+	573	WP_007493433.1	YcxB family protein	-
GXM20_RS00760	145134..146561	+	1428	WP_007493431.1	sigma 54-interacting transcriptional regulator	-
GXM20_RS00765	146846..148246	+	1401	WP_007493429.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	131956..165649	33693

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (66-170)

Antitoxin

• Protein of unknown function DUF6718 (1-74)

Sequences

Toxin

Protein

• Length: 206 a.a.
• Molecular weight: 23674.46 Da
• Isoelectric Point: 5.0818

>T149449 WP_039997549.1 NZ_CP048436:142591-143208 [Flavonifractor plautii]
MAEDVLALNGLSIELLTQLSYRSQLGLSLKRTLRHFEREALIAELVSMTEWLDEQTGLSEIALDYRIKSYASIVGKYERY
FNSSCHVSQVFNDVLGFRAFCDSYNSVLALTSTSFSVADFSRGKAVDDGYRGVHLYFQLDNNHYPIEIQFNTLYDRQLNN
WLHDYLYKKTYSNEVGQQLRARYENGKIRNQSEFEEVLNDVLSGG

Nucleotide

• Length: 618 bp

>T149449 NZ_CP064103:c4307510-4307196 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCACACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8643.02 Da
• Isoelectric Point: 9.4481

>AT149449 WP_007493440.1 NZ_CP048436:143189-143419 [Flavonifractor plautii]
MCYLVAKDTEKRGCYALKTTHGERLVNLKRALDQEVGVKGIQLVTISRPTAYGEYAPYTFAKDEQEFKTLVRAMRA

Nucleotide

• Length: 231 bp

>AT149449 NZ_CP064103:c4307746-4307513 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Y4FFD1