Detailed information of TA system TA149447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2653085..2653614
• Replicon: chromosome
• Accession: NZ_CP048431
• Organism: Staphylococcus aureus strain SA1428

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GZ067_RS13025
• Protein ID: WP_000621175.1
• Coordinates: 2653252..2653614 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: GZ067_RS13020
• Protein ID: WP_000948331.1
• Coordinates: 2653085..2653255 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZ067_RS12990	2648121..2648681	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
GZ067_RS12995	2648890..2649369	+	480	WP_001287079.1	hypothetical protein	-
GZ067_RS13000	2649362..2650945	+	1584	WP_164026399.1	PH domain-containing protein	-
GZ067_RS13005	2650932..2651423	+	492	WP_001205912.1	PH domain-containing protein	-
GZ067_RS13010	2651427..2651786	+	360	WP_000581197.1	holo-ACP synthase	-
GZ067_RS13015	2651852..2653000	+	1149	WP_001281154.1	alanine racemase	-
GZ067_RS13020	2653085..2653255	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
GZ067_RS13025	2653252..2653614	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GZ067_RS13030	2653964..2654965	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
GZ067_RS13035	2655084..2655410	+	327	WP_001052491.1	anti-sigma factor antagonist	-
GZ067_RS13040	2655412..2655891	+	480	WP_179302320.1	anti-sigma B factor RsbW	-
GZ067_RS13045	2655866..2656636	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T149447 WP_000621175.1 NZ_CP048431:2653252-2653614 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T149447 NZ_CP064103:3887778-3887996 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT149447 WP_000948331.1 NZ_CP048431:2653085-2653255 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT149447 NZ_CP064103:3887378-3887752 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1