Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4962231..4962856 Replicon chromosome
Accession NZ_CP048430
Organism Klebsiella pneumoniae strain KP18-3-8

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GZ213_RS25220 Protein ID WP_002882817.1
Coordinates 4962473..4962856 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GZ213_RS25215 Protein ID WP_004150355.1
Coordinates 4962231..4962473 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZ213_RS25190 4958222..4958821 + 600 WP_004151865.1 glucose-1-phosphatase -
GZ213_RS25195 4958815..4959675 + 861 WP_002882807.1 virulence factor BrkB family protein -
GZ213_RS25200 4959672..4960109 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GZ213_RS25205 4960154..4961095 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GZ213_RS25210 4961109..4962026 - 918 WP_002882812.1 alpha/beta hydrolase -
GZ213_RS25215 4962231..4962473 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GZ213_RS25220 4962473..4962856 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GZ213_RS25225 4963030..4963959 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GZ213_RS25230 4963956..4964591 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GZ213_RS25235 4964588..4965490 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T149433 WP_002882817.1 NZ_CP048430:4962473-4962856 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T149433 NZ_CP064103:227624-227731 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT149433 WP_004150355.1 NZ_CP048430:4962231-4962473 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT149433 NZ_CP064103:c227575-227512 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References