Detailed information of TA system TA149429

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3972792..3973446
• Replicon: chromosome
• Accession: NZ_CP048430
• Organism: Klebsiella pneumoniae strain KP18-3-8

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R4YHX2
• Locus tag: GZ213_RS20115
• Protein ID: WP_004144731.1
• Coordinates: 3972792..3973199 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: GZ213_RS20120
• Protein ID: WP_002916312.1
• Coordinates: 3973180..3973446 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZ213_RS20095	3968792..3970525	-	1734	WP_004151783.1	single-stranded-DNA-specific exonuclease RecJ	-
GZ213_RS20100	3970531..3971244	-	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
GZ213_RS20105	3971267..3972163	-	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
GZ213_RS20110	3972264..3972785	+	522	WP_002916308.1	flavodoxin FldB	-
GZ213_RS20115	3972792..3973199	-	408	WP_004144731.1	protein YgfX	Toxin
GZ213_RS20120	3973180..3973446	-	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
GZ213_RS20125	3973692..3974675	+	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
GZ213_RS20130	3974826..3975485	-	660	WP_002916317.1	hemolysin III family protein	-
GZ213_RS20135	3975649..3975960	-	312	WP_002916319.1	N(4)-acetylcytidine aminohydrolase	-
GZ213_RS20140	3976010..3976738	+	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
GZ213_RS20145	3976857..3978290	+	1434	WP_002916322.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15936.69 Da
• Isoelectric Point: 11.4778

>T149429 WP_004144731.1 NZ_CP048430:c3973199-3972792 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNAE
WEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Nucleotide

• Length: 408 bp

>T149429 NZ_CP064096:2289975-2290721 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT149429 WP_002916312.1 NZ_CP048430:c3973446-3973180 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT149429 NZ_CP064096:2290721-2290891 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A378E4P6

Antitoxin

• AlphaFold DB: A0A0H3GY41