Detailed information of TA system TA149417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3333508..3334049
• Replicon: chromosome
• Accession: NZ_CP048427
• Organism: Rhizobium daejeonense strain KACC 13094

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GT349_RS19100
• Protein ID: WP_163904233.1
• Coordinates: 3333508..3333831 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GT349_RS19105
• Protein ID: WP_163906182.1
• Coordinates: 3333828..3334049 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GT349_RS19085	3329177..3330373	-	1197	WP_163904229.1	GGDEF domain-containing protein	-
GT349_RS19090	3330583..3332748	-	2166	WP_186663216.1	PBP1A family penicillin-binding protein	-
GT349_RS19095	3332998..3333462	+	465	WP_163904231.1	YcgN family cysteine cluster protein	-
GT349_RS19100	3333508..3333831	-	324	WP_163904233.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GT349_RS19105	3333828..3334049	-	222	WP_163906182.1	antitoxin MazE family protein	Antitoxin
GT349_RS19110	3334367..3336805	+	2439	WP_163906184.1	heavy metal translocating P-type ATPase	-
GT349_RS19115	3336904..3337320	+	417	WP_163904235.1	Cu(I)-responsive transcriptional regulator	-
GT349_RS19120	3337407..3337613	+	207	WP_163904237.1	heavy-metal-associated domain-containing protein	-
GT349_RS19125	3337948..3338298	+	351	WP_163904239.1	carboxymuconolactone decarboxylase family protein	-
GT349_RS19130	3338388..3338738	+	351	WP_163904240.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3327001..3339657	12656

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (7-67)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11267.11 Da
• Isoelectric Point: 7.0124

>T149417 WP_163904233.1 NZ_CP048427:c3333831-3333508 [Rhizobium daejeonense]
MKRGDLVTVAVSGDFGKPRPALVVQADAFEGTGTVTVLLITSTLVEAPLIRPSIEPNAGNGLSKPSQIMVDKALSVRRER
IGPTIGHIDDETMLSVTRSLALFLGIA

Nucleotide

• Length: 324 bp

>T149417 NZ_CP064095:c3496732-3496553 [Bacillus subtilis]
ATGTCGACCTATGAATCTCTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATTCATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8020.01 Da
• Isoelectric Point: 5.0789

>AT149417 WP_163906182.1 NZ_CP048427:c3334049-3333828 [Rhizobium daejeonense]
VATSISKRVEKRRMALRAAGLRPVQIWLPDTRRPGFADECRRQSLAVAASDRSDPDLGNLLDEVLAESGEDDA

Nucleotide

• Length: 222 bp

>AT149417 NZ_CP064095:3496407-3496629 [Bacillus subtilis]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACCACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCTAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAAAAGAAACTACCCTTTAATAG
GAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTTC