Detailed information of TA system TA149413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 36971..37768
• Replicon: plasmid pA_CitB
• Accession: NZ_CP048417
• Organism: Citrobacter freundii strain CitB

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: GZ999_RS22950
• Protein ID: WP_171279078.1
• Coordinates: 37265..37768 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: GZ999_RS22945
• Protein ID: WP_016582626.1
• Coordinates: 36971..37240 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZ999_RS22915	32694..33419	-	726	WP_171279049.1	hypothetical protein	-
GZ999_RS22920	33612..34370	-	759	WP_171279050.1	DUF2971 domain-containing protein	-
GZ999_RS22925	34480..34890	-	411	WP_060458854.1	hypothetical protein	-
GZ999_RS22930	34880..35524	-	645	WP_060458853.1	hypothetical protein	-
GZ999_RS22935	35526..35879	-	354	WP_171279077.1	hypothetical protein	-
GZ999_RS22940	35885..36550	-	666	WP_000161333.1	AAA family ATPase	-
GZ999_RS22945	36971..37240	+	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
GZ999_RS22950	37265..37768	+	504	WP_171279078.1	GNAT family N-acetyltransferase	Toxin
GZ999_RS22955	37797..38003	-	207	WP_171279051.1	hypothetical protein	-
GZ999_RS22960	37954..38205	-	252	WP_000147960.1	hypothetical protein	-
GZ999_RS22965	38207..38899	-	693	WP_171279052.1	hypothetical protein	-
GZ999_RS22970	38913..39236	-	324	WP_000064173.1	hypothetical protein	-
GZ999_RS22975	39423..40007	-	585	WP_171279053.1	tail fiber assembly protein	-
GZ999_RS22980	40861..42513	-	1653	Protein_50	tail fiber protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110347	110347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18612.31 Da
• Isoelectric Point: 8.8885

>T149413 WP_171279078.1 NZ_CP048417:37265-37768 [Citrobacter freundii]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVHQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T149413 NZ_CP064094:3466344-3466595 [Bacillus subtilis]
ATGAACTTCTCCTTTAGTTCCTACCCATATTATAACATGATCAAGCACATTGCAAACATGAAACGATTCTCATTATGGTT
TACCCATATCACATTCATTGGCTTATTCTTAATGTTTCAACTCATCAAAGATTACTTCAGCAGCGAAGGACAGGCGCTAA
TCAACACAATTTTTGTTGTCACATGTATCATTGCCATATTGTTATGGATCATCTATTGTGTATTCCTTAAACTAAGAAAC
AAGTCACACTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT149413 WP_016582626.1 NZ_CP048417:36971-37240 [Citrobacter freundii]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT149413 NZ_CP064094:c3466366-3466266 [Bacillus subtilis]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3